Determination of bioequivalence of lomefloxacin tablets using urinary excretion data

Journal of Pharmaceutical and Biomedical Analysis
Shailesh A ShahDharmesh B Patel


The present study describes development of a sensitive and simple HPTLC method for estimation of lomefloxacin (LMF) in human urine. The drug was extracted using chloroform after adjusting the pH of urine to 7.0. Chloroform extract was spotted on silica gel 60 F(254) TLC plate and was developed in a mixture of n-butanol-methanol-ethyl acetate-6 M ammonia (4:2:3:2, v/v/v/v) as the mobile phase and scanned at 290 nm. The peak for LMF resolved at R(F) of 0.40+/-0.02. The method was validated in terms of linearity (50-600 microgram/ml), precision, specificity and accuracy. The limit of detection and limit of quantification for LMF in urine were found to be 20 and 50 microgram/ml, respectively. The average recovery of LMF from urine was 91.93%. The proposed method was applied to generate urinary excretion data for LMF after administration of two market LMF tablet formulations (400 mg, Formulation R and Formulation T) to six healthy human volunteers in a two-treatment, open, crossover design. Various pharmacokinetic parameters like peak excretion rate ((dAU/dt)(max)), time for peak excretion rate (t(max)), AUC(0-48), AUC(0- infinity ), cumulative amount and % cumulative amount of LMF excreted, elimination half-life (t(1/2)), terminal ...Continue Reading


Apr 1, 1989·The Journal of Antimicrobial Chemotherapy·C Y ChanG L French
Sep 1, 1989·The Journal of Antimicrobial Chemotherapy·D J Griggs, R Wise
Apr 1, 1984·Journal of Pharmaceutical Sciences·B K MartinJ McEwen
Oct 27, 2004·Journal of Learning Disabilities·S D SmithR K Olson


Dec 2, 2006·Chemical & Pharmaceutical Bulletin·Maissa Yacoub SalemLaila El-Sayed Abd-El-Fattah

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