PMID: 11337049May 5, 2001Paper

Determination of bovine adenovirus-3 titer based on immunohistochemical detection of DNA binding protein in infected cells

Journal of Virological Methods
Y ZhouSuresh Kumar Tikoo

Abstract

DNA sequence coding for a portion of DNA binding protein (amino acids 3-58) of bovine adenovirus type-3 (BAV-3) was cloned and expressed in Escherichia coli as a fusion protein with Schistosoma japonicum glutathione S-transferase. The fusion protein was affinity purified and used to immunize rabbits. Immunoprecipitation and Western blot analysis showed that the antiserum could specifically recognize a protein of 48 kDa in BAV-3-infected cells, which was produced both in early and late phases of BAV-3 life cycle. Based on the ability of antiserum to recognize DNA binding protein, a novel assay for BAV-3 quantitation was established. The assay is less time consuming and can be performed on a wide variety of bovine cells. In addition, virus titers determined by this assay are comparable to the standard plaque assay.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Jan 1, 1986·Annual Review of Biochemistry·J W Chase, K R Williams
Jul 2, 1966·Nature·J H Darbyshire

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Citations

May 29, 2004·Virology·Vikas KulshreshthaSuresh K Tikoo
Mar 17, 2017·Journal of Virology·Amit GabaSuresh Tikoo
Mar 11, 2006·The Journal of General Virology·Amrutlal K Patel, Suresh K Tikoo
Jan 26, 2021·Frontiers in Microbiology·Xin Zhao, Suresh K Tikoo
Dec 17, 2020·Viruses·Sugandhika KhosaSuresh K Tikoo

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