PMID: 7033476Oct 1, 1981Paper

Determination of brain enolase isozymes with an enzyme immunoassay at the level of single neurons

Journal of Neurochemistry
K KatoR Semba

Abstract

Ultrasensitive enzyme immunoassay systems for the assay of rat brain enolase isozymes (alpha alpha, alpha gamma, and gamma gamma forms) were prepared by use of beta-D-galactosidase from Escherichia coli as label and the purified rabbit antibodies to alpha alpha and gamma gamma enolases. The antibodies were purified from the immunoglobulin G (IgG) fractions of antisera by immunoaffinity chromatography with a column of the corresponding antigen-coupled Sepharose. Sandwich-type immunoassay systems with the galactosidase-labeled antibody Fab' fragments and the antibody F(ab')2-immobilized polystyrene beads could determine amounts as small as 1 amol (10(-18) mol) of each isozyme. Purkinje cell bodies picked up from the bulk-separated fraction by means of a nylon loop were subjected to the assay at the level of single cells. In contrast to previous report, this neuron contained not only the gamma gamma but also the alpha gamma and alpha alpha enolases at a level of amol per cell body, although the concentration of gamma gamma was the highest. Immunohistochemical experiments on te cerebellum with the peroxidase-labeled antirabbit IgG antibody and the unlabeled antibody method confirmed the above results, and indicated that both alpha ...Continue Reading

References

Feb 23, 1976·Biochemical and Biophysical Research Communications·P J MarangosC York
Jan 20, 1978·Science·D SchmechelF K Goodwin
Aug 31, 1977·Experimental Brain Research·J Altman, S A Bayer
Nov 8, 1976·Biochimica Et Biophysica Acta·L FletcherC B Taylor
Sep 1, 1975·Archives of Biochemistry and Biophysics·P J MarangosC York
Mar 1, 1981·Journal of Neurochemistry·K KatoY Umeda
Jun 1, 1980·Journal of Biochemistry·F SuzukiK Kato

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Citations

May 20, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y IshiguroM Nagaya
Feb 7, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·K KatoY Ariyoshi
Apr 13, 1984·Clinica Chimica Acta; International Journal of Clinical Chemistry·T ShimizuK Kato
Dec 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversG Rijksen
Sep 30, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y Y ZhuK Kato
Jul 1, 1987·Cell Differentiation·Y TsutsuiK Kato
Jul 1, 1990·Brain Research. Developmental Brain Research·R SembaK Kato
Apr 27, 1984·Biochimica Et Biophysica Acta·A Shimizu, K Kato
Jan 1, 1982·Neurochemistry International·H ScarnaJ F Pujol
Aug 6, 1982·Biochimica Et Biophysica Acta·A ShimizuK Kato
Sep 2, 1991·Biochimica Et Biophysica Acta·Y InagumaK Kato
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E A HaanW M Cowan
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·G B KoelleG Boulla
Jul 1, 1992·World Journal of Surgery·O OzakiK Toshima
Aug 1, 1994·Journal of Neuroscience Research·A KellerM Lucas
Aug 13, 1990·Brain Research·E AokiS Kashiwamata
Nov 25, 1985·Brain Research·J S Meyer, G N Joy
Dec 1, 1983·Journal of Neurochemistry·M J KornblattL Legault-Demare
Oct 18, 2005·Biochemical and Biophysical Research Communications·Lirlândia P SousaCláudio A Bonjardim
Nov 26, 1986·Biochemical and Biophysical Research Communications·M LazarL Legault-Demare
Sep 16, 1991·Biochemical and Biophysical Research Communications·N OchiK Kato
Apr 1, 1990·Journal of Neurochemistry·K KatoT Sato

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