Determination of carbohydrate profiles of Bacillus anthracis and Bacillus cereus including identification of O-methyl methylpentoses by using gas chromatography-mass spectrometry.

Journal of Clinical Microbiology
Alvin FoxS Rostovtseva

Abstract

Bacillus anthracis and Bacillus cereus are closely related pathogenic organisms that are difficult to differentiate phenotypically or genotypically. It is well known that vegetative and spore forms of bacilli are quite distinct both morphologically and chemically, but spore-specific chemical markers allowing these species to be distinguished have not been previously described. By using gas chromatography-mass spectrometry, vegetative cells and spores of the two species were shown to exhibit distinct carbohydrate profiles. Profiles of vegetative B. anthracis typically contained high levels of galactose but did not contain galactosamine, whereas B. cereus contained galactosamine and generally low levels of galactose. Spore cultures exhibited unique carbohydrate profiles compared with those of vegetative cultures. B. anthracis spore profiles contained rhamnose alone, whereas B. cereus spore profiles contained rhamnose and fucose. Additionally, two spore-specific O-methylated methylpentoses were discovered. Both B. anthracis and B. cereus spores contained 3-O-methyl rhamnose, whereas B. cereus spores also contained 2-O-methyl rhamnose. Carbohydrate profiling is demonstrated to be a powerful tool for differentiating the two closely ...Continue Reading

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Citations

Dec 14, 2001·The Journal of Biological Chemistry·Christina SchäfferPaul Messner
May 20, 2003·Journal of Bacteriology·Sarah J ToddAnne Moir
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Feb 28, 2016·The Journal of Biological Chemistry·Emmanuel MaesYann Guerardel
Jun 5, 2003·Journal of Microbiological Methods·Alvin FoxRobert L Price
Dec 8, 2004·Carbohydrate Research·Olga TarasenkoKalle Levon
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Apr 20, 2010·Carbohydrate Research·Herwig StepanErika Staudacher

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