Determination of carnitine and acylcarnitines in human urine by means of microextraction in packed sorbent and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Sylwia Magiera, Jacek Baranowski

Abstract

A method using semi-automatic microextraction by packed sorbent (eVol®-MEPS) and hydrophilic interaction chromatography-ultra-high-performance liquid chromatography-tandem mass spectrometry (HILIC-UHPLC-MS/MS) was described for the simultaneous determination of carnitine and acylcarnitines in human urine. The optimal conditions of MEPS extraction were obtained using C2 of M1 (C8+SCX) phase as a sorbent. Chromatographic separation of the analytes was achieved within 2.5min on Acquity UPLC BEH HILIC column using a gradient elution program with water containing 5mM ammonium acetate and acetonitrile as the mobile phase. The detection was performed on a triple-quadrupole tandem mass spectrometer in a positive ion mode via electrospray ionization (ESI). The linearity of the calibration curves for all compounds was found over a range from 0.1ng/mL to 500ng/mL. The method afforded satisfactory results in terms of sensitivity, specificity, precision, accuracy, recovery as well as stability of the analyte under various conditions. The method was used successfully for determination of carnitine and acylcarnitines in human urine.

References

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Feb 16, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sylwia MagieraJacek Baranowski
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Citations

Oct 27, 2015·Journal of Chromatography. a·Francesco IadarestaMohamed Abdel-Rehim
Sep 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Olga FilippouVictoria Samanidou
Nov 12, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liu YangMohamed Abdel-Rehim
Jan 18, 2018·Critical Reviews in Analytical Chemistry·Samin HamidiAliasghar Hamidi
Mar 9, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xi XuXin Gao
Jul 23, 2021·Food Science of Animal Resources·Jung Min ParkJin Man Kim

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