Determination of carprofen in blood by gas chromatography chemical ionization mass spectrometry

Biomedical Mass Spectrometry
B J HodshonG J Bader


A gas chromatographic chemical ionization mass spectrometric assay has been developed to measure carprofen in blood. The method features the addition of either a structural or stable isotope analog internal standard to plasma prior to a simple benzene extraction at pH 4.5. The residue, after removal of the benzene, is methylated with ethereal diazomethane. Following evaporation of the methylating solvents, a portion of the reconstituted residue is analyzed by gas chromatography mass spectrometry. The mass spectrometer is set to monitor in the gas chromatographic effluent the [MH]+ ions of carprofen methyl ester and the methyl ester of the internal standard generated by isobutane chemical ionization. Assay sensitivity is 5 pmol ml-1. When 200 pmol ml-1 samples are analyzed using a stable isotope analog as the internal standard, the precision and accuracy are both 4%. Using a structural analog as the internal standard, the assay was neither as precise nor as accurate.


Oct 1, 1977·Clinical Pharmacology and Therapeutics·J S DutcherA J Atkinson

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Sep 1, 1984·Journal of Pharmaceutical Sciences·A J RossD L Casey
Jul 1, 1986·Journal of Clinical Pharmacology·W A Garland, M P Barbalas
Sep 1, 1981·Biomedical Mass Spectrometry·I Horman

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