Determination of catecholamines in rat heart tissue and plasma samples by liquid chromatography with electrochemical detection

Journal of Chromatography
B M Eriksson, B A Persson

Abstract

Liquid chromatography with electrochemical detection is used for the determination of adrenaline, noradrenaline and dopamine in rat heart tissue, and the method has also been applied to the determination of basic levels of these compounds in blood plasma. The catecholamines are isolated from the biological sample by adsorption onto alumina and are then desorbed by elution with perchloric acid. The stability of the compounds during the different stages in the work-up process has been studied. A greatly simplified procedure for the preparation of alumina is presented. Both ion-pair reversed-phase and ion-exchange liquid chromatography have been used for the separation of the catecholamines. For plasma samples the method has been validated against radioenzymatic assay and the choice of method is discussed.

References


❮ Previous
Next ❯

Citations

Sep 1, 1996·Biomedical Chromatography : BMC·G AlemanyA Gamundi
Jan 1, 1990·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·P LindblomT Holmin
Nov 1, 1987·Neurochemical Research·A MorettiF Trunzo
Jan 1, 1990·Journal of Neural Transmission. General Section·M HeiligE Widerlöv
Feb 8, 1985·Biochimica Et Biophysica Acta·P HanssonP Nilsson-Ehle
Sep 11, 1987·European Journal of Pharmacology·M InoueM Fujiwara
Nov 10, 1987·European Journal of Pharmacology·M InoueM Fujiwara
Jul 23, 1991·European Journal of Pharmacology·G BaoT Unger
Aug 15, 1983·Life Sciences·S E Shoaf, M A Elchisak
Oct 1, 1989·Pharmacology, Biochemistry, and Behavior·T D SteeleG K Yim
Apr 1, 1988·Psychiatry Research·R G LaessleK M Pirke
Jul 1, 1989·Brain Research. Developmental Brain Research·P Gil-Loyzaga, N Parés-Herbute
Jan 1, 1987·Comparative Biochemistry and Physiology. A, Comparative Physiology·F VillarroyaT Mampel
Mar 1, 1994·Comparative Biochemistry and Physiology. Comparative Physiology·R W Rosebrough
Mar 31, 1995·Forensic Science International : Synergy·J Hirvonen, P Huttunen
Jan 1, 1985·Experimental Gerontology·N CarfagnaA Moretti
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N Parés-HerbutéH Astier
Jan 1, 1987·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·F Ehrenström, P Johansson
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R Fløysand, G Serck-Hanssen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.