PMID: 2503559Jul 26, 1989Paper

Determination of cell-bound Fc receptors using a cell adherence test (CAT)

Journal of Immunological Methods
H LemkeH Hansen

Abstract

The determination of membrane-bound Fc receptors as rosette forming cells with antibody-coated erythrocytes (EA-RFC) is a tedious and time consuming method. Here, we describe a modification of the assay delineated by Eisenbarth et al. (J. Immunol. Methods (1980) 39, 387) for the detection of Fc receptors on cell surfaces. V-shaped microtiter plates were coated with an antigen (e.g., fluorescein isothiocyanate (FITC) coupled to bovine serum albumin (BSA] and subsequently with monoclonal anti-FITC antibodies of different isotypes. Cells to be tested were added, incubated for a short time or centrifuged immediately. Cells expressing FcR, became bound to the flanks of the V-shaped wells, whereas cells lacking the receptor were pelleted to the tip of the well. This cell adherence test (CAT) is independent of the agglutinating abilities of the antibodies, and since it is very fast and highly reproducible, large numbers of different cells can be screened for FcR expression. Anti-receptor antibodies can also be tested for their binding to FcR.

References

Sep 1, 1979·The Journal of General Virology·A F BradburneR R Coombs
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Jan 1, 1976·Journal of Immunological Methods·J W Goding
Jul 1, 1976·European Journal of Immunology·G Köhler, C Milstein
Jul 16, 1987·Journal of Immunological Methods·R R CoombsM P Cranage
Feb 11, 1985·Journal of Immunological Methods·D M WyattG D Street
Jan 1, 1985·Transfusion·L T SinorF V Plapp
Jan 1, 1985·Transfusion·J M RachelF V Plapp
Apr 1, 1974·The Journal of Experimental Medicine·G Möller
Dec 1, 1980·Molecular Immunology·J D RodwellV N Schumaker
Jan 1, 1980·Journal of Immunological Methods·G S EisenbarthA S Fauci

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.