Determination of cell capacitance using the exact empirical solution of partial differential Y/partial differential Cm and its phase angle

Biophysical Journal
J Santos-Sacchi

Abstract

Measures of membrane capacitance offer insight into a variety of cellular processes. Unfortunately, popular methodologies rely on model simplifications that sensitize them to interference from inevitable changes in resistive components of the traditional cell-clamp model. Here I report on a novel method to measure membrane capacitance that disposes of the usual simplifications and assumptions, yet is immune to such interference and works on the millisecond timescale. It is based on the exact empirical determination of the elusive partial derivative, partial differential Y/partial differential C(m), which heretofore had been approximated. Furthermore, I illustrate how this method extends to the vesicle fusion problem by permitting the determination of partial differential Y(v)/partial differential C(v), thereby providing estimates of fusion pore conductance and vesicle capacitance. Finally, I provide simulation examples and physiological examples of how the method can be used to study processes that are routinely interrogated by measures of membrane capacitance.

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Citations

Apr 7, 2007·The Journal of Membrane Biology·Takahisa AbeHideichi Shinkawa
Mar 8, 2006·Pflügers Archiv : European journal of physiology·Joseph Santos-SacchiDhasakumar Navaratnam
Dec 3, 2008·Pflügers Archiv : European journal of physiology·Hao ZhangAnlian Qu
Jun 21, 2006·Annals of Biomedical Engineering·Pavel Novák, Ivan Zahradník
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lei Song, Joseph Santos-Sacchi
Feb 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anthony J RicciJoseph Santos-Sacchi
Sep 20, 2015·The European Journal of Neuroscience·Srividya SundaresanMirna Mustapha
Nov 11, 2015·The American Journal of Pathology·Sharen E McKayGerald S Shadel
Aug 23, 2005·Biophysical Journal·Dhasakumar NavaratnamJoseph Santos-Sacchi
Nov 22, 2005·Biophysical Journal·Enrique G Navarrete, Joseph Santos-Sacchi
Feb 10, 2010·Biophysical Journal·Lei Song, Joseph Santos-Sacchi
Jul 29, 2008·Biophysical Journal·Volodymyr Rybalchenko, Joseph Santos-Sacchi
Mar 27, 2012·Communicative & Integrative Biology·Michael E SchneeAnthony J Ricci
Apr 23, 2009·Biophysical Journal·Jun-Ping BaiDhasakumar Navaratnam
Jan 27, 2009·Biophysical Journal·Joseph Santos-SacchiLei Song
Jun 9, 2016·Biophysical Journal·Joseph Santos-Sacchi, Lei Song
Oct 6, 2017·Journal of Neurophysiology·Lauren A PoppiAlan M Brichta
Nov 23, 2018·Nature·Lauren ChessumRonna Hertzano
Apr 17, 2014·Visual Neuroscience·Karlene M Cork, Wallace B Thoreson
Jan 16, 2008·Physiological Reviews·Jonathan Ashmore
Feb 21, 2014·The Journal of Biological Chemistry·Joseph Santos-Sacchi, Lei Song
Mar 27, 2015·Journal of Neurophysiology·Yohan SongJohn S Oghalai
Apr 27, 2010·The Journal of Biological Chemistry·Jun-Ping BaiDhasakumar Navaratnam
Apr 12, 2020·Scientific Reports·Joseph Santos-Sacchi, Winston Tan
Mar 17, 2021·Journal of Neural Engineering·William G A BrownPaul R Stoddart

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