Determination of cell surface charge by photometric titration

Journal of Biochemical and Biophysical Methods
K ThethiM Duszyk

Abstract

A colloid titration method has been frequently used to determine the number of charged residues at the cell surface. Here we present a new version of this technique, based on photometric measurements of a metachromatic shift in the maximum absorption of toluidine blue as it binds to the cell surface. The major improvements are: (1) simplified methodology and (2) increased precision of equivalence point determination. The data are analyzed using Gran's theory, which allows measurements to be taken at regular intervals instead of being concentrated around the equivalence titration point. We used this method to characterize the cell surface charge of three populations of rat mast cells: (1) peritoneal mast cells (PMC), (2) bone marrow-derived mast cells (BMMC) and (3) a rat cultured mast cell line (RCMC). Our results indicate that PMC have (4.23 +/- 0.59) x 10(8), while BMMC (8.58 +/- 0.26) x 10(7) negatively charged residues per cell. The results for RCMC were similar to those for BMMC. Taking into account the size differences between PMC and BMMC, the average charge density of PMC was also significantly higher than that of BMMC. The differences in cell surface charge were analyzed in the light of different sensitivities of mast ...Continue Reading

References

Aug 1, 1992·Analytical Biochemistry·M P Van DammeB N Preston
May 1, 1992·Biophysical Journal·S Marcelja
Jan 1, 1986·International Archives of Allergy and Applied Immunology·H AliJ C Foreman
Dec 1, 1971·The Journal of General Physiology·S G McLaughlinG Eisenman
Feb 1, 1984·Journal of General Microbiology·Y Ando, T Tsuzuki
Jan 1, 1994·Biophysical Journal·D NaranjoM F Schumaker

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Citations

Nov 30, 2010·The Journal of the Acoustical Society of America·Ozkan Doganay, Yuan Xu
Apr 7, 2012·IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control·Ozkan Doganay, Yuan Xu
Dec 25, 2004·Biophysical Chemistry·James P Camp, Adam T Capitano
Jul 17, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Akinori UedaKazuo Tanishita
Jan 23, 2010·Journal of Biomaterials Science. Polymer Edition·Ivan DjordjevicDavid A Steele

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