PMID: 6163485Jan 22, 1981Paper

Determination of cell/medium interfacial tensions from contact angles in aqueous polymer systems

Biochimica Et Biophysica Acta
S SchurchD J McIver

Abstract

The contact angles on cell layers of a series of polymeric droplets from aqueous two-phase systems of dextran and poly(ethylene glycol) have been used to determine the critical or limiting interfacial tension for spreading on the cell layers. Test droplets of the denser dextran-rich phase were formed in the lighter poly(ethylene glycol)-rich phase. The interfacial tensions, gamma, between the phases were determined with the pendant drop method, and a linear relationship was found between gamma-1/2 and the cosine of the angle the droplets made with the cell layers (Good-Girifalco plot). We were thus able to determine the limiting or critical interfacial tension, gamma c, for spreading on the cell layers. The value of gamma c is a measure of the interfacial energy of the cell/bathing medium interface. Values of gamma c obtained by this method include the following: 0.65 and 0.84 microN . m-1 for human erythrocytes and neutrophils, respectively; 0.93 microN . m-1 for porcine pulmonary macrophages; 0.75--3.60 microM . m-1 for various transformed murine lymphoid cell lines, and 2.53 microN . m-1 for Balb/c murine spleen lymphocytes. Exposure to various agents has differing effects on gamma c. Concanavalin A reduces gamma c, and bact...Continue Reading

References

Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J R LuckasenJ H Kersey

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Citations

Dec 1, 1989·Experientia·C KrekelerJ Klein
Dec 1, 1986·Prostaglandins, Leukotrienes, and Medicine·N A PatersonS Schurch
Apr 29, 1998·Journal of Cataract and Refractive Surgery·C M CunananP M Knight
Jul 1, 1984·Microvascular Research·M J DunlopC P Taylor
Jan 1, 1982·Annals of the New York Academy of Sciences·W J SibbaldF S Schurch
Aug 1, 1988·Cell Biophysics·B Mely-GoubertD F Gerson
Feb 13, 2020·Physical Chemistry Chemical Physics : PCCP·Amber R TitusBoris Y Zaslavsky
Apr 1, 1988·Journal of Biomedical Materials Research·D R AbsolomG Chang
May 14, 1985·Biochimica Et Biophysica Acta·S A JohnstoneE R Tustanoff

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