Determination of choline and ethanolamine plasmalogens in human plasma by HPLC using radioactive triiodide (1-) ion (125I3-)

Analytical Biochemistry
Ryouta Maeba, Nobuo Ueta

Abstract

For the purpose of developing highly sensitive and convenient determination of plasmalogens, the high-performance liquid chromatography (HPLC) method using radioactive iodine ((125)I) was investigated. Radioactive triiodide (1-) ion ((125)I(3)(-)), which is an actual iodine form capable of reacting with vinyl ether bond ([bond]CH(2)[bond]O[bond]CH[double bond]CH[bond]) of plasmalogens, could be safely and efficiently produced by oxidizing a commercial radioactive sodium iodine (Na(125)I) with hydrogen peroxide (H(2)O(2)) under acid condition (pH 5.5-6.0), which is called iodine-125 reagent. I(3)(-) specifically reacted with plasmalogens at the molar ratio of 1:1 in methanol, and 1 or 2 mol of plasmalogens was involved in the binding with iodine per iodine atom, resulting in the formation of stable iodine-binding phospholipids. The HPLC system with Diol column and acetonitrile/water as a mobile phase was available for separating iodine-binding phospholipids from nonbinding free iodine and for separately eluting iodine-binding phospholipids derived from choline and ethanolamine plasmalogens. Using iodine-125 reagent (1.85 MBq/ml), plasmalogens were detectable at high sensitivity of 10,000-15,000 cpm/nmol, which is more than 1000-...Continue Reading

References

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Feb 27, 2003·Biochemical and Biophysical Research Communications·Ryouta Maeba, Nobuo Ueta
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Mar 3, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jacqueline LessigJürgen Arnhold

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Citations

Apr 5, 2011·Analytical and Bioanalytical Chemistry·Shu-Ping HuiTakao Kurosawa
Dec 19, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ryouta MaebaYoshinori Fujiwara
Jul 30, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Megumi NishimukaiHiroshi Hara
Mar 3, 2007·Journal of Atherosclerosis and Thrombosis·Ryouta MaebaTamio Teramoto

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