PMID: 3262687Oct 4, 1988Paper

Determination of circulating immune complexes by chicken anti-human C3 and anti-human C1q microELISA

Journal of Immunological Methods
A LarssonJ Sjöquist

Abstract

The present study reports on an anti-human C1q and an anti-human C3 microELISA for measuring circulating immune complexes (CIC). Affinity-purified chicken anti-human C3 and anti-human C1q were used as capture antibodies and protein A-alkaline phosphatase conjugate for detection. Chicken antibodies do not activate mammalian complement, do not react with rheumatoid factor and are not bound to anti-mammalian IgG antibodies or protein A, which often are used for detection in ELISA. They are therefore suitable as capture antibodies in CIC assays. We have tested Bell's palsy patients and found an increase in both anti-C3- and anti-C1q-containing CIC in acute and convalescent sera compared with the normal.

References

Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G W Miller, V Nussenzweig
Apr 6, 1988·Journal of Immunological Methods·A Larsson, J Sjöquist
Jan 1, 1987·ORL; Journal for Oto-rhino-laryngology and Its Related Specialties·L JonssonL Thomander
Jan 1, 1967·Archiv für die gesamte Virusforschung·R Gispen, B Brand-Saathof
Feb 1, 1971·European Journal of Biochemistry·R Axén, S Ernback
Jan 1, 1981·Annual Review of Medicine·R C Williams
Jan 1, 1981·Scandinavian Journal of Immunology·A LundwallJ Sjöquist

Related Concepts

Immune complex
Blood
Complement Activating Enzymes
Complement component C1
Complement C3 precursor
Enzyme-Linked Immunosorbent Assay
Hemifacial Paralysis
Polyglobin
Immune Sera
Microchemistry

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