PMID: 25752052Mar 11, 2015Paper

Determination of clopidogrel sulfate in pharmaceutical formulation and biological fluids by extraction spectrofluorimetric method

Guang pu xue yu guang pu fen xi = Guang pu
Wei-Qin LianXiashi Zhu

Abstract

A simple and sensitive spectrofluorimetric method has been developed for the determination of clopidogrel sulfate in pharmaceutical formulation and human urine/serum. It is based on the formation of ion-pair complex between clopidogrel sulfate and alizarin red in 0.3 mol x L(-1) hydrochloric acid solution. The ion pair complex was extracted in dichloromethane and the fluorescence intensity was measured at 550 nm after excitation at 428 nm. The various factors influencing ion-pair complex formation and fluorescence determination were discussed. Under the optimized conditions, the fluorescence value showed a good linear relationship with the clopidogrel sulfate concentration ranging from 1.0 to 11.0 μg x mL(-1). The equation of calibration curve was F = 53.32 + 35.01c (μg x mL(-1)), r = 0.994, and the detection limit was found to be 0.11 μg x mL(-1). No interference was observed from common co-existing substances or pharmaceutical excipient. The determination recoveries of clopidogrel sulfate in pharmaceutical formulation and human urine/serum samples were 90.6%-99.3%, 104.6%-109.3%, 96.3%-105.0%, respectively. The method was successfully applied to detect clopidogrel sulfate in clopidogrel sulfate tablet and human urine/ serum. ...Continue Reading

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