PMID: 16523704Mar 10, 2006Paper

Determination of concentration and aggregate size in influenza virus preparations using the true UV-absorption spectra

Molekuliarnaia biologiia
A L KsenofontovEugenie N Dobrov

Abstract

It is well-known that influenza virus (IV) preparations are characterized by very large contribution of light-scattering to their UV absorption spectra. With the help of so called extrapolation method we managed to measure true absorption spectra of IV preparations and to determine absorption coefficients (E0.1(1) (cm, 280)) for the intact IV virions and for IV subviral particles. These coefficients turned out to equal 1.26 +/- 0.17 and 0.96 +/- 0.11 for the virions and subviral particles respectively. The knowledge of exact IV concentration is necessary for quantitative physico-chemical studies of IV virions and their components. It is also shown that UV absorption spectra measurements allow to register IV virion aggregation. Aggregation properties of IV subviral particles were also studied.

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Citations

Oct 1, 2011·Molekuliarnaia biologiia·A L KsenofontovL A Baratova
Jul 12, 2013·PloS One·Alexander L KsenofontovEugeny N Dobrov
Apr 9, 2015·Journal of Biomolecular Structure & Dynamics·Alexander L KsenofontovEugeny N Dobrov
Aug 1, 2012·Biotechnology Progress·Petiot Emma, Amine Kamen
May 26, 2017·Journal of Biomolecular Structure & Dynamics·Alexander L KsenofontovEleonora V Shtykova
Jun 10, 2017·Environmental Science & Technology·Charles P Gerba, Walter Q Betancourt

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