Determination of copper, zinc and iron in broncho-alveolar lavages by atomic absorption spectroscopy

Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)
C HarlykA van der Eeckhout

Abstract

Concentrations of Zn, Cu and Fe were measured in 157 broncho-alveolar lavages (BAL), before and after centrifugation, collected at the Leuven University Hospital (Belgium). Zn was measured by flame-atomic absorption spectroscopy, using direct calibration, while Cu and Fe were determined by electrothermal atomic absorption spectroscopy, using the method of standard additions. For Fe only 56 samples were measured. Most of the studied elements are present in the liquid phase (supernatant). About 90% of Cu concentrations lie between 0 and 15 micrograms/kg, while 90% of Zn concentrations are lower than 230 micrograms/kg, with 30% between 30 and 70 micrograms/kg, and 50% between 100 and 200 micrograms/kg. There seems to be a reverse relationship between Cu and Zn levels with high Cu going along with low Zn and vice versa.

References

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Citations

May 22, 2010·Analytical and Bioanalytical Chemistry·Seydou YaoCyril Petibois
Jul 31, 2008·Biological Trace Element Research·E BargagliP Rottoli
Aug 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina M PaninaMikhail S Gelfand
May 9, 2013·Molecular Microbiology·Sulman ShafeeqTomas G Kloosterman
Sep 1, 2018·Metallomics : Integrated Biometal Science·Belén Callejón-LeblicTamara García-Barrera
Oct 31, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Abiodun D OgunniyiJames C Paton
Dec 16, 2017·Journal of Breath Research·Andrew J GhioCharles R Esther
Jan 27, 2009·Journal of Bacteriology·Shashi ChillappagariMohamed A Marahiel
Feb 15, 2019·International Journal of Molecular Sciences·Belén Callejón-LeblicTamara García-Barrera

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