Determination of cuticular and internal fatty acids of Chorthippus brunneus males and females using HPLC-LLSD and GC-MS

Biomedical Chromatography : BMC
Marek GołebiowskiPiotr Stepnowski

Abstract

Insects are of growing significance in veterinary medicine and human healthcare; therefore, an understanding of their biology is very important. The cuticular and internal fatty acid compositions of Chorthippus brunneus males and females have been studied for the first time. The lipids of males and females were separated into classes of compounds using high-performance liquid chromatography with a laser light scattering detector. The free fatty acid (FFA) fractions obtained by HPLC were silylated and then analyzed by GC-MS. The cuticular lipids of males contained 15 saturated, four unsaturated with even-numbered and two unsaturated with odd-numbered carbon chains, FFAs ranging from C8 to C25. The major free fatty acids in males were C16 (20.8%), C18:2 (8.5%), C18:1 (32.9%) and C18 (24.4%). The cuticular lipids of females contained 17 saturated, four monounsaturated and two diunsaturated free fatty acids ranging from C8 to C30. The major cuticular fatty acids in females were C16 (25.1%), C18:2 (6.2%), C18:1 (23.7%) and C18:0 (33.2%). The internal FFAs of males consisted of 20 compounds ranging from C8 to C26. Four of these compounds were detected as major compounds: C16 (14.1%), C18:2 (21.6%), C18:1 (38.0%) and C18 (22.5%). Amon...Continue Reading

References

Nov 1, 1987·Archives of Environmental Contamination and Toxicology·B A HunterD J Thompson
May 19, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·D R NelsonJ S Buckner
Nov 1, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Maria AugustyniakMichał Augustyniak
Jan 11, 2007·The Journal of Experimental Biology·Joshua B Benoit, David L Denlinger
Mar 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Marek GołebiowskiPiotr Stepnowski
May 3, 2007·Biomedical Chromatography : BMC·Leah N Williamson, Michael G Bartlett
Aug 2, 2007·Analytical and Bioanalytical Chemistry·C De PasqualeS Colazza
Nov 26, 2009·Journal of Insect Physiology·Marek GołebiowskiPiotr Stepnowski
Jun 14, 2012·The Journal of Experimental Biology·Marek GołębiowskiPiotr Stepnowski
Sep 12, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Magdalena CerkowniakMarek Gołębiowski
Jul 1, 1989·Journal of Chemical Ecology·K E Espelie, E A Bernays
Dec 3, 2014·Journal of Pharmaceutical and Biomedical Analysis·Magdalena Buszewska-ForajtaRoman Kaliszan

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