Determination of Cyanide by Microdiffusion Technique Coupled to Spectrophotometry and GC/NPD and Propofol by Fast GC/MS-TOF in a Case of Poisoning

Journal of Analytical Toxicology
Gabriella RodaVeniero Gambaro

Abstract

A man was found dead in a hotel located near Rome (Italy). The man was still holding a syringe attached to a butterfly needle inserted in his left forearm vein. The syringe contained a cloudy pinkish fluid. In the hotel room the Police found a broken propofol glass vial plus four sealed ones, an opened NaCl plastic vial and six more still sealed, and a number of packed smaller disposable syringes and needles. An opened plastic bottle containing a white crystalline powder labeled as potassium cyanide was also found. Systematic toxicological analysis (STA), carried out on blood, urine and bile, evidenced only the presence of propofol in blood and bile. So the validated L-L extraction protocol and the GC/MS-TOF method for the confirmation of propofol in the biological fluids optimized in our laboratory was applied to blood, urine and bile. The concentration of propofol resulted to be 0.432 μg/mL in blood and 0.786 μg/mL in bile. The quantitative determination of cyanide in blood was carried out by microdiffusion technique coupled to spectrophotometric detection obtaining a cyanide concentration of 5.3 μg/mL. The quantitative determination was then confirmed by GC/NPD and the concentration of cyanide resulted to be 5.5 μg/mL in blo...Continue Reading

References

Sep 1, 1983·Journal of Analytical Toxicology·F McAuley, D S Reive
May 25, 1994·Forensic Science International : Synergy·T C ChaoT H Koh
Jul 1, 1994·Journal of Analytical Toxicology·M OdoulP Chambon
May 1, 1994·Journal of Analytical Toxicology·M LaforgeP Levillain
Sep 16, 1998·Journal of Analytical Toxicology·D Felscher, M Wulfmeyer
Sep 24, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·S ChinakaK Ueda
Jun 29, 2000·Journal of Analytical Toxicology·A Cruz-LandeiraM J Tabernero-Duque
May 18, 2001·International Journal of Legal Medicine·S Iwersen-BergmannA Schmoldt
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Antonia M Calafat, Stephen B Stanfill
Apr 6, 2005·Journal of Analytical Toxicology·Pierre DumasAlain LeBlanc
May 12, 2005·Forensic Science International : Synergy·Masataka SakayanagiYoshihiro Harigaya
Jan 25, 2006·Forensic Science International : Synergy·Elena F KraniotiManolis Michalodimitrakis
Aug 30, 2007·Journal of Analytical Toxicology·Elodie SaussereauChristian Lacroix
Sep 18, 2007·Fundamental & Clinical Pharmacology·Anne RoussinMaryse Lapeyre-Mestre
May 24, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wolfram MiekischJochen K Schubert
Jun 2, 2009·Journal of Forensic and Legal Medicine·Gabriella KlauszKlára Töro
Dec 10, 2013·Forensic Science International : Synergy·Eunyoung HanHeesun Chung
Apr 15, 2014·Journal of Analytical Toxicology·Veniero GambaroPaolo Procaccianti
Oct 5, 2017·Journal of Analytical Toxicology·Paolo ProcacciantiVeniero Gambaro

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Citations

Jan 29, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gabriella RodaMichele Dei Cas

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