PMID: 15385841Sep 24, 2004Paper

Determination of cyclosporine in saliva using liquid chromatography-tandem mass spectrometry

Therapeutic Drug Monitoring
Anisha E MendonzaFatemeh Akhlaghi

Abstract

Saliva may offer an alternative specimen for the therapeutic monitoring of cyclosporine (CsA) in children and patients with difficult venous access. For a highly protein-bound drug such as CsA, saliva may also provide a practical approach for measuring the unbound concentration. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is ideally suited for the measurement of drugs in saliva. A solid-phase extraction technique, analytic liquid chromatography over an Aqua Perfect C18 column, maintained at 65 degrees C, and electrospray tandem mass spectrometry were used to quantify CsA in saliva. The method used cyclosporine C (CsC) as the internal standard. Mobile phase comprised of a 97:3 vol/vol mixture of methanol and 30 mmol ammonium acetate at a flow rate of 0.5 mL/min. Chromatograms using mass transitions of m/z 1219.9 --> m/z 1202.9 for CsA and m/z 1235.9 --> m/z 1218.9 for CsC were obtained. The calibration curve was linear from 1 to 300 microg/L with correlation coefficient values ranging from 0.9732 to 0.9968). The lower limit of quantification was 1 microg/L, and limit of detection was 0.6 microg/L with an average extraction recovery of 84.7 +/- 2.6% for CsA and 93.7 +/- 4.4% for CsC from the saliva matrix. The accur...Continue Reading

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Citations

May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Oct 26, 2010·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Vladimir BelostotskyNicholas J A Webb
Sep 15, 2010·Indian Journal of Pharmaceutical Sciences·F AzizM F Khan
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhi-gang FangQiang Zhang
Aug 15, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Irene van den BroekJos H Beijnen
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Oct 10, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Amitava Dasgupta
Oct 25, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mwlod Ghareeb, Fatemeh Akhlaghi
Jun 17, 2006·Therapeutic Drug Monitoring·Anisha E MendonzaFatemeh Akhlaghi
Mar 8, 2005·Journal of Mass Spectrometry : JMS
Sep 12, 2019·Bioanalysis·Lucía Paniagua-GonzálezAna de-Castro-Ríos
Jun 12, 2018·BioMed Research International·Farah Asa'adGiovanni Damiani

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