Determination of cysteine by capillary zone electrophoresis with end-column amperometric detection at a gold/mercury amalgam microelectrode without deoxygenation

Journal of Chromatography. a
W Jin, Y Wang

Abstract

Capillary zone electrophoresis was employed for the determination of cysteine using an end-column amperometric detection with a gold/mercury amalgam microelectrode, at a constant potential of 0.02-0.06 V vs. saturated calomel electrode. In this procedure deoxygenation is not necessary. The electrochemical characteristics at the microelectrode, the effect of the concentration of the buffer and the separation voltage across the capillary on the migration time and separation efficiency, and the dependence of the injection voltage and time on the detection signal, the separation efficiency and coulometric efficiency has been investigated. The calibration plot was found to be linear over four orders of magnitude and the limit of detection was 5.8 x 10(-8) mol/l (or 14.5 amol). The method was applied to the determination of cysteine in human plasma, blood and urine.

Citations

May 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei WangWenrui Jin
Apr 12, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ali A EnsafiHassan Karimi-Maleh
Oct 13, 2012·Biosensors & Bioelectronics·Hailong LiErkang Wang
Aug 31, 2016·Scientific Reports·Maryam Shahrajabian, M Reza Hormozi-Nezhad
Jun 24, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Zezhou YangXiaofeng Lu
Feb 24, 2016·Chemical Communications : Chem Comm·Zhifeng MaoDik-Lung Ma
Aug 16, 2014·Chemistry : a European Journal·Mainak GangulyTarasankar Pal
May 3, 2008·Luminescence : the Journal of Biological and Chemical Luminescence·Amir WaseemAbdul Nabi
Jan 21, 2014·Analytical and Bioanalytical Chemistry·Sihua Qian, Hengwei Lin

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