PMID: 9188832May 9, 1997Paper

Determination of D-fenfluramine, D-norfenfluramine and fluoxetine in plasma, brain tissue and brain microdialysate using high-performance liquid chromatography after precolumn derivatization with dansyl chloride

Journal of Chromatography. B, Biomedical Sciences and Applications
P ClausingJ F Bowyer

Abstract

A HPLC method is described for the simultaneous determination of D-fenfluramine (FEN), D-norfenfluramine (NF) and fluoxetine (FLX) using fluorometric detection after precolumn derivatization with dansyl-chloride. The method has limits of quantitation of 200 fmol for FEN and NF, 500 fmol for FLX in brain microdialysate, and 1 pmol for NF and FEN, and 2 pmol for FLX in plasma. Brain tissue standards were linear between 5 and 200 pmol/mg for all three compounds. The inter-assay variability (relative standard deviation) was 6.6%, 6.9% and 9.3% for FEN, 4.6%, 3.7% and 7.9% for NF and 10.4%, 4.9% and 12.2% for FLX, for brain microdialysate (2 pmol/microl), plasma (2 pmol/ microl) and brain tissue (50 pmol/mg), respectively. Intra-assay variability was always lower, typically several times lower than inter-assay variability. Extraction recovery was 108% and 48% for FEN, 105% and 78% for NF and 94% and 45% for FLX, in plasma (2 pmol/microl) and brain tissue (5 pmol/mg), respectively. Due to the stability of the dansyl-chloride derivatives this method is well suited for an autoinjector after manual derivatization with dansyl chloride at room temperature for 4 h.

References

Jan 19, 1990·Journal of Chromatography·S H WongH Kranzler
Jul 1, 1968·Journal of Pharmaceutical Sciences·R B Bruce, W R Maynard
Apr 1, 1994·Journal of Chromatography. B, Biomedical Applications·J N ZengH H Stuting

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Citations

Oct 9, 2002·Journal of Pharmaceutical and Biomedical Analysis·Mohamed A El-dawyFawzy A El-Barbary
Sep 3, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·M KarabacakN Sundaraganesan
Jan 13, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xuejun KangZhongze Gu
Feb 23, 2008·Critical Reviews in Clinical Laboratory Sciences·Sarah M R WilleWilly E E Lambert
Jun 5, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J C AlvarezO Spreux-Varoquaux

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