PMID: 8468383Feb 26, 1993Paper

Determination of dapoxetine, an investigational agent with the potential for treating depression, and its mono- and di-desmethyl metabolites in human plasma using column-switching high-performance liquid chromatography

Journal of Chromatography
C L Hamilton, J D Cornpropst

Abstract

A column-switching high-performance liquid chromatographic (HPLC) method is described for the determination of dapoxetine and its mono- and di-desmethyl metabolites in human plasma. The analytes, including an internal standard, were extracted from plasma at basic pH with hexane-ethyl acetate. The organic extract was evaporated to dryness and the residue reconstituted with acetonitrile. The analytes were separated from late-eluting endogenous substances on a Zorbax RX-C8 pre-column. The front-cut fraction containing the analytes was further separated on a second RX-C8 column. The analytes were detected by their native fluorescence, using excitation and emission wavelengths of 230 and 330 nm, respectively. The limit of quantitation was determined to be 20 ng/ml, and the response was linear from 20 to 200 ng/ml. The method has been successfully applied to human plasma samples in a Phase I study.

Citations

Apr 2, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tae Kon KimHye Hyun Yoo
May 6, 2014·Journal of Pharmaceutical and Biomedical Analysis·András DarcsiSzabolcs Béni
Feb 15, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Thomas Y HsuehTung-Hu Tsai
Mar 29, 2008·The Journal of Sexual Medicine·Marcel D Waldinger, Dave H Schweitzer
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Robert SkibińskiMaciej Gawlik

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