Determination of diffusion coefficients of proteins in beaded agarose by gel filtration.

Journal of Chromatography
P A Davies

Abstract

A method for determining approximately the coefficient of diffusion of solute molecules in beaded gels, based on the analysis of breakthrough curves obtained in gel filtration, is described. This is applied to the diffusion of porcine serum proteins, gamma-globulin and fibrinogen in Sepharose CL-4B. The diffusion coefficients at 20 degrees C are 11.2 and 3.6 microns 2/s, respectively, about half those calculated from the partition coefficients using published equations.

Citations

May 20, 1996·Biotechnology and Bioengineering·R H LiF T Gentile
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·W C Lee, C H Chen
Feb 14, 2006·The Journal of Physical Chemistry. B·Magnus SchröderJürgen Hubbuch

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