PMID: 9188813May 9, 1997Paper

Determination of dimethylated arginines in human plasma by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
A PetterssonV Backman

Abstract

Using high-performance liquid chromatography (HPLC) with multigradient elution, N(G),N(G)-dimethyl-L-arginine (asymmetric-DMA, ADMA) and N(G),N'(G)-dimethyl-L-arginine (symmetric-DMA, SDMA) can be separated from human plasma samples. The dimethylarginine compounds in plasma, after extraction with a cation-exchange column, are converted to fluorescent derivatives with o-phthaldialdehyde (OPA) in an alkaline medium and the derivatives are separated simultaneously within 50 min on a reversed-phase column (Ultracarb 3 ODS(20)). The recoveries of ADMA and SDMA are over 80% and the method permits quantitative determination of dimethylated arginines at concentrations as low as 0.1 micromol/l in human plasma.

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Citations

Oct 7, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Hillevi BlomsterHannu Uusitalo
Dec 23, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dimitrios TsikasJan T Kielstein
Oct 8, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C L GopuK Sreenivasan
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Dec 4, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Friedrich SchulzeRainer H Böger
Oct 4, 2005·Clinical Chemistry and Laboratory Medicine : CCLM·Tom Teerlink
Aug 23, 2005·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Jan T Kielstein, Carmine Zoccali
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tamila HeresztynJohn D Horowitz
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·John D Horowitz, Tamila Heresztyn
Aug 26, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tom Teerlink
Sep 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jens Martens-Lobenhoffer, Stefanie M Bode-Böger
Apr 1, 2009·Expert Opinion on Drug Metabolism & Toxicology·Carol Wadham, Arduino A Mangoni
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