PMID: 9171394Jun 1, 1997Paper

Determination of DNA sequences required for regulated Mycobacterium tuberculosis RecA expression in response to DNA-damaging agents suggests that two modes of regulation exist

Journal of Bacteriology
F MovahedzadehE O Davis

Abstract

The recA gene of Mycobacterium tuberculosis has previously been cloned and sequenced (E. O. Davis, S. G. Sedgwick, and M. J. Colston, J. Bacteriol. 173:5653-5662, 1991). In this study, the expression of this gene was shown to be inducible in response to various DNA-damaging agents by using a transcriptional fusion to the reporter gene encoding chloramphenicol acetyltransferase. A segment of DNA around 300 bp upstream of the coding region was shown to be required for expression. However, primer extension analysis indicated that the transcriptional start sites were 47 and 93 bp upstream of the translation initiation codon. Sequence motifs with homology to two families of Escherichia coli promoters but also with significant differences were located near these proposed transcription start sites. The differences from the E. coli consensus patterns would explain the previously described lack of expression of the M. tuberculosis recA gene from its own promoter in E. coli. In addition, the M. tuberculosis LexA protein was shown to bind specifically to a sequence, GAAC-N4-GTTC, overlapping one of these putative promoters and homologous to the Bacillus subtilis Cheo box involved in the regulation of SOS genes. The region of DNA 300 bp up...Continue Reading

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Citations

Dec 12, 2001·Microbiology·K G PapavinasasundaramE O Davis
Nov 2, 2002·Molecular Microbiology·Elaine O DavisErik C Böttger
Apr 24, 2009·FEMS Microbiology Reviews·Tiago Dos VultosBrigitte Gicquel
Jun 27, 2014·BioMed Research International·Mariana Noelia VialeMaría Isabel Romano
Oct 27, 2017·Scientific Reports·Begonia Fudrini OlivenciaFrank Imkamp
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R VenkateshK Muniyappa
Nov 28, 2007·Journal of Bacteriology·Jong-Hee LeeWilliam R Bishai
Oct 4, 2003·Journal of Bacteriology·Krishna K GopaulElaine O Davis
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Hrvoje PetkovićPaul F Long
Jan 28, 1999·Journal of Bacteriology·Z FangK J Forbes
May 9, 1998·Journal of Bacteriology·M D Bashyam, A K Tyagi
Nov 26, 1997·Journal of Bacteriology·J A Gonzalez-y-MerchandR A Cox
Nov 26, 1998·Journal of Bacteriology·A Tapias, J Barbé
Jan 1, 1997·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M A MulderL M Steyn

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