Determination of Drugs in Plasma Samples by High-Performance Liquid Chromatography-Tandem Mass Spectrometry for Therapeutic Drug Monitoring of Schizophrenic Patients

Journal of Analytical Toxicology
Diego S DominguesMaria Eugênia Costa Queiroz

Abstract

This work describes the development of a simple, sensitive and selective method based on high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) to determine antipsychotics (olanzapine, quetiapine, clozapine, haloperidol and chlorpromazine) along with antidepressants (mirtazapine, paroxetine, citalopram, sertraline, imipramine, clomipramine and fluoxetine), anticonvulsants (carbamazepine and lamotrigine) and anxiolytics (diazepam and clonazepam) in plasma samples obtained from schizophrenic patients. The samples were prepared by protein precipitation. The target drugs were separated on an XSelect SCH C18 column (100 mm × 2.1 mm × 2.5 µm) within 8.0 min by means of gradient elution. The drugs were then detected on a quadrupole tandem mass spectrometer equipped with an electrospray ionization source, operating in the multiple reactions monitoring mode and in the positive ionization mode. The LC-MS-MS method was linear range from subtherapeutic to toxic concentrations with lower limit of quantification values ranged from 0.2 to 5.0 ng mL(-1), precision with coefficient of variation values lower than 12%, and accuracy ranged from 90 to 108%. The developed method enabled successful analysis of the targe...Continue Reading

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Citations

Jan 18, 2020·Journal of Chromatographic Science·Daniel da Mota Castelo BrancoDavi Pereira de Santana
Aug 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ewelina Dziurkowska, Marek Wesolowski
Sep 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tomasz Tuzimski, Anna Petruczynik
Nov 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karina Sommerfeld-KlattaFranciszek K Główka

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