Determination of Egg Number Added to Special Pasta by Means of Cholesterol Contained in Extracted Fat Using GC-FID

Foods
D NaviglioMonica Gallo

Abstract

Pasta with eggs added (generally termed "special pasta" for Italian legislation) is made by adding no less than 4 eggs without shells (or no less than 200 g of liquid or lyophilized egg product) per kilogram of semolina, as provided by law. In this work, to determine the final content of eggs added to dough, an analytical procedure was developed for the rapid analysis of the cholesterol content in the finished pastas. The proposed procedure was simpler, faster, and more accurate than that of official methods of analysis based on the gravimetric determination of sterols. Moreover, the determination of the quality of fat content in the special pasta (egg pasta in this case) allowed the evaluation of its origin, avoiding possible fraud resulting from the addition of foreign fat as an alternative to fat derived from eggs. In this new gas chromatographic procedure, the internal standard squalene for the quantification of cholesterol was used because a more polar GC capillary column was used (RTX 65 TG-HT) for the separation of sterols, rather than 5% phenyl methylsilicone. The ratio between cholesterol and squalene allowed for the determination of the number of eggs added, while from analysis of the same gas chromatogram, it was als...Continue Reading

References

Jan 6, 2004·The Journal of Nutrition·Kristin L Herron, Maria Luz Fernandez
Jun 11, 2010·The American Journal of Clinical Nutrition·Luc DjousséKenneth J Mukamal
Sep 11, 2010·Critical Reviews in Food Science and Nutrition·Tina Fuad, P Prabhasankar
Jul 22, 2011·Food & Function·Maria Luz Fernandez
Jun 2, 2012·Journal of AOAC International·Thu T N DinhMallory L Boylan
Sep 18, 2014·Journal of Mass Spectrometry : JMS·Dania Al-BalaaHelena Čížková

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