Determination of embryonic polarity in a regulative system: evidence for endogenous inhibitors acting sequentially during primitive streak formation in the chick embryo

Development
Federica BertocchiniClaudio D Stern

Abstract

Avian embryos have a remarkable capacity to regulate: when a pre-primitive streak stage embryo is cut into fragments, each fragment can spontaneously initiate formation of a complete embryonic axis. We investigate the signalling pathways that initiate primitive streak formation and the mechanisms that ensure that only a single axis normally forms. As reported previously, an ectopic primitive streak can be induced by misexpression of Vg1 in the marginal zone. We now show that Vg1 induces an inhibitor that travels across the embryo (3 mm distance) in less than 6 hours. We provide evidence that this inhibitor acts early in the cascade of events downstream of Vg1. We also show that FGF signalling is required for primitive streak formation, in cooperation with Nodal and Chordin. We suggest that three sequential inhibitory steps ensure that a single axis develops in the normal embryo: an early inhibitor that spreads throughout the embryo (which can be induced by Vg1), a second inhibition by Cerberus from the underlying hypoblast, and finally a late inhibition from Lefty emitted by the primitive streak itself.

References

Jan 1, 1981·Anatomy and Embryology·C D Stern, G W Ireland
Jan 1, 1997·Annual Review of Cell and Developmental Biology·R Harland, J Gerhart
Mar 30, 1999·Mechanisms of Development·T A YatskievychP B Antin
Dec 10, 1999·Mechanisms of Development·Y IshimaruS Noji
Jun 1, 2001·Nature·Y Chen, A F Schier
Aug 30, 2001·Current Biology : CB·L Kodjabachian
Aug 15, 2002·Mechanisms of Development·Isaac Skromne, Claudio D Stern
Oct 3, 2002·Nature Cell Biology·Patrick P L Tam
Nov 8, 2002·Development·Karolina Piotrowska, Magdalena Zernicka-Goetz
Nov 15, 2002·Developmental Cell·Federica Bertocchini, Claudio D Stern
Nov 19, 2002·Critical Reviews in Eukaryotic Gene Expression·Leo A van GrunsvenKristin Verschueren
Dec 10, 2002·Reproductive Biomedicine Online·R L Gardner
Dec 25, 2002·Current Biology : CB·Yu Chen, Alexander F Schier
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Jun 28, 2003·FEBS Letters·An ZwijsenDanny Huylebroeck
Sep 27, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard L Gardner, Timothy J Davies
Oct 23, 2003·Annual Review of Cell and Developmental Biology·Alexander F Schier
Dec 1, 1969·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·P D Nieuwkoop
Dec 1, 1969·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·P D Nieuwkoop

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Citations

Jun 14, 2005·Brain Research. Brain Research Reviews·Paula Alexandre, Marion Wassef
Apr 8, 2006·Nature Biotechnology·Evan Y SnyderMichael W Kalichman
Dec 2, 2011·Development·Timothy J Stuhlmiller, Martín I García-Castro
May 21, 2010·PloS One·Bakhtier VasievCornelis J Weijer
Apr 19, 2006·Nature Reviews. Genetics·David Kimelman
Mar 28, 2007·Nature Reviews. Genetics·Patrick P L Tam, David A F Loebel
Jan 16, 2007·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jody A White, Janet Heasman
Dec 12, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Laura J WagstaffAndrea Münsterberg
Feb 20, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Guojun Sheng
Jun 24, 2006·Current Opinion in Genetics & Development·Claudio D Stern
Oct 10, 2013·Stem Cell Research·Sittipon Intarapat, Claudio D Stern
Mar 25, 2008·Mechanisms of Development·Irene de AlmeidaClaudia Linker
Feb 20, 2007·Mechanisms of Development·Anne Grapin-Botton, Daniel Constam
Jan 28, 2015·Developmental Biology·Matthew J Birket, Christine L Mummery
May 27, 2006·Developmental Biology·Manli ChuaiCornelis J Weijer
Nov 25, 2006·Developmental Biology·Ann C FoleyMark Mercola
May 21, 2016·Scientific Reports·Satoshi IshishitaYoichi Matsuda
Jun 9, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Federica Bertocchini, Susana M Chuva de Sousa Lopes
Feb 9, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Matthew J Stower, Federica Bertocchini
Nov 19, 2017·Scientific Reports·Clemente F AriasFederica Bertocchini
Feb 28, 2020·Open Biology·Hyung Chul LeeClaudio D Stern
Feb 9, 2007·Development·Michael M Shen
Jun 21, 2020·Mechanisms of Development·Guillermo Serrano Nájera, Cornelis J Weijer

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