Determination of enkephalin peptides by nonaqueous capillary electrophoresis with electrochemical detection

Electrophoresis
Arndt PsurekGerhard K E Scriba

Abstract

Nonaqueous capillary electrophoresis with electrochemical detection (NACE-ED) was applied to the analysis of enkephalin peptides. The effect of different buffer compositions on the electrophoretic behavior of methionine enkephalin, leucine enkephalin, and [D-Ala2]-leucine enkephalin was studied. Separation of the protonated and the deprotonated peptides was obtained using ACN/methanol-based electrolyte systems. The electrochemical behavior of the enkephalins was studied by the capillary batch injection analysis technique. NACE-ED yielded well-defined signals in the oxidation mode only for the negatively charged analytes. The optimized BGE for the counterelectroosmotic separation consisted of 10 mM ammonium acetate in ACN/methanol (3:1 v/v). Using a platinum microdisk electrode set to an actual potential of +0.65 V detection limits in the submicromolar range were observed which are about one order of magnitude lower compared to UV detection. Problems concerning EOF instability and electrode fouling caused by water and other neutral sample impurities transported by the EOF can be avoided in the EOF-inverted mode using poly(ethylene glycol)-coated capillaries and an actual working electrode potential of +1.0 V. For the quantificat...Continue Reading

References

Dec 1, 1989·Analytical Chemistry·A M Warner, S G Weber
Feb 10, 1998·Electrophoresis·P D Voegel, R P Baldwin
Oct 25, 2000·Journal of Chromatography. a·M L RiekkolaI E Valkó
Feb 28, 2001·Fresenius' Journal of Analytical Chemistry·U BackofenC E Lunte
Nov 15, 2002·Electrophoresis·Lisa A Holland, Alyison M Leigh
Dec 14, 2002·Electrophoresis·Marja-Liisa Riekkola
May 14, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wei WangWenrui Jin
Jul 15, 2005·Journal of Chromatography. a·Veronika SolínováSalvatore Fanali

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Citations

Jun 27, 2007·The Analyst·Grégoire Herzog, Damien W M Arrigan
Dec 25, 2008·Electrophoresis·Laurent Geiser, Jean-Luc Veuthey
Nov 30, 2007·Electrophoresis·Václav Kasicka

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