Determination of enoxacin using Tb composite nanoparticles sensitized luminescence method

Journal of Fluorescence
Mohammad Mainul Karim, Sang Hak Lee

Abstract

In our study, terbium-acetylacetone (Tb-acac) composite nanoparticles have been prepared under vigorous ultrasonic irradiation. The nanoparticles are water soluble, stable and have extremely narrow emission bands and high internal quantum efficiencies. They were used as fluorescence probes in the determination of enoxacin (Enox) based on the fluorescence enhancement of nanoparticles through fluorescence resonance energy transfer (FRET). The influence of buffer solution on the fluorescence intensity was investigated. Under the optimum conditions, the fluorescence intensity of the Tb-acac-Enox system is linearly proportional to the Enox concentration in the Enox concentration range of 2 x 10(-7)-1 x 10(-4) M. The correlation coefficient for the calibration curve was 0.9976. The limit of detection as defined by IUPAC, C (LOD) = 3S (b)/m (where S (b) is the standard deviation of the blank signals and m is the slope of the calibration graph) was found to be 3 x 10(-8) M. The relative standard deviation (RSD) for six repeated measurements of 1 x 10(-4) M Enox was 1.35%. The method was applied to the determination of Enox in pharmaceutical formulation and recovery results were obtained from urine samples.

References

Jul 20, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·M HernándezM Calull
Mar 14, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ning ZhangYu Liu
Jul 12, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A Espinosa-MansillaF Salinas
Aug 16, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shi-lv ChenHui-chun Zhao
Feb 24, 2006·Journal of Fluorescence·Mohammad Mainul KarimSang Beom Hong
May 12, 2007·Journal of Fluorescence·Mohammad Mainul KarimSang Hak Lee
Aug 29, 2007·Analytica Chimica Acta·Mohammad Mainul KarimSang Hak Lee

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Citations

Dec 21, 2013·Macromolecular Rapid Communications·Xuepeng ZhangGuoqing Zhang
Mar 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ana-Mădălina MăciucăValentina Uivarosi

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