Determination of erythrocyte pyrimidine 5'-nucleotidase activity by 31P nuclear magnetic resonance: comparison of normal controls and multiple sclerosis patients

Experientia
G L MendzP A Crossie

Abstract

A technique to assay erythrocyte pyrimidine 5'-nucleotidase activity in situ using 31P nuclear magnetic resonance spectroscopy is presented. The assay is chemically specific, simple and applicable to untreated lysates. A comparison of enzyme levels in normal controls and in multiple sclerosis patients employing the assay yielded no significant differences between both groups. Difficulties encountered in the quantitative analysis of the assay using 1H-NMR spectroscopy are briefly discussed.

References

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Oct 1, 1974·The Journal of Clinical Investigation·W N ValentineW S Adams
Mar 1, 1980·British Journal of Haematology·J E Lieberman, E C Gordon-Smith
Jan 1, 1981·Enzyme·J D Torrance, D A Whittaker
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M S SwansonR S Markin
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Citations

May 15, 2004·Progress in Biophysics and Molecular Biology·Philip W Kuchel
Aug 5, 2000·Baillière's Best Practice & Research. Clinical Haematology·J L Vives i Corrons
Jun 14, 2017·Multiple Sclerosis Journal - Experimental, Translational and Clinical·Kira GroenJeannette Lechner-Scott
Mar 26, 2019·Human Antibodies·Farzad KobarfardSoudeh Ghafouri-Fard

Related Concepts

Erythrocytes
Multiple Sclerosis, Acute Fulminating
In Vivo NMR Spectroscopy
Nucleotidases
5'-NUCLEOTIDASE

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