Determination of ethambutol in plasma using selected ion monitoring

Journal of Chromatography
K OhyaM Sano

Abstract

The determination of ethambutol in plasma is described. Using ethambutol-d4 as an internal standard, ethambutol and the internal standard were extracted with chloroform under alkaline conditions, and converted into their trifluoroacetyl derivatives with trifluoroacetic anhydride in benzene-pyridine (4:1). Selected ion monitoring was carried out by monitoring the peaks at m/z 294 and 296 corresponding to the fragment ion [M/2]+ of the derivatives. Ethambutol was determined by use of the peak height ratio of the peak at m/z 294 against that at m/z 296. The method was utilized for studying the bioavailability and pharmacokinetics of the drug.

References

May 1, 1974·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J A Burton, L S Schanker

Citations

Jan 1, 1984·Journal of Pharmaceutical and Biomedical Analysis·A Hulshoff, H Lingeman

Related Concepts

Metazoa
Biological Availability
Deuterons
Canis familiaris
Etambutol Llorente
Ions
Chromatography, Gas-Liquid-Mass Spectrometry
Standardization

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.