PMID: 11312817Apr 21, 2001Paper

Determination of ETU in tomatoes and tomato products by HPLC-PDA: evaluation of cleanup procedures

Journal of Agricultural and Food Chemistry
S KontouC Tzia

Abstract

An HPLC-PDA method for the determination of ethylenethiourea (ETU), the main degradation product of the organic fungicides ethylene bis(dithiocarbamate)s (EBDCs), in tomatoes and tomato products is reported. Solid-matrix liquid-liquid (l-l) partitioning and separatory funnel l-l partitioning for the cleanup were examined. The effect of salt addition, pH, and phase ratio on analyte recovery at the cleanup step was studied. It was found that solid-matrix l-l partitioning afforded higher precision and more selective separation of the analyte. According to the method proposed, the samples were extracted with methanol/water (3:1, v/v) and cleaned up on an Extrelut 20 column. ETU was eluted with dichloromethane and separated on a reversed phase HPLC column. For tomato products with degrees Brix > 20 further purification through silica cartridge was adopted. The method was validated over the following ranges of concentrations: 0.01-0.5 mg/kg for tomatoes, 0.01-0.1 mg/kg for tomato juice, and 0.05-0.25 mg/kg for tomato paste. The accuracy (recoveries > 70%) and the precision obtained (%RSD < 10%) were satisfactory.

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Citations

Jul 9, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Gül Ozhan, Buket Alpertunga
Apr 12, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·A BonnechèreJ van Loco
Apr 6, 2011·Biomedical Chromatography : BMC·Bina GuptaRahul Kumar
Jun 17, 2006·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E D CaldasP E Boon
May 23, 2018·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Narong Chamkasem
Mar 24, 2018·Environmental Science and Pollution Research International·Dennis Guilherme Costa-SilvaJeferson Luis Franco
Jul 31, 2020·Analytical Methods : Advancing Methods and Applications·Siriwan NantapholWeena Siangproh
Mar 5, 2004·Journal of Agricultural and Food Chemistry·Stella KontouConstantina Tzia

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