PMID: 19938513Nov 27, 2009Paper

Determination of five biogenic amines in source water by ion chromatography

Se pu = Chinese journal of chromatography
Xinying ZhaoJinghua Zhang

Abstract

An ion chromatographic (IC) method was developed for the separation of five biogenic amines (BAs), including putrescine, cadaverine, histamine, spermidine and spermine, in source water. The sample was separated on a TSK-GEL SuperIC cation exchange column (150 mm x 4.6 mm) after filtering treatment by 0.45 microm inorganic membrane. Methanesulfonic acid was used as eluent for concentration gradient elution at the rate of 1.0 mL/min. The injection volume was 100 microL and the BAs were detected by a non-suppressed conductivity detector. The result showed that the baseline separation was obtained for the 5 kinds of BAs. The linear relationships between the concentration and the peak area were obtained in the concentration range of 1.0 - 30.0 mg/L. The relative standard deviations (RSDs) of retention time and peak area were no more than 0.02% and 2.08%, respectively. The recoveries were from 96.0% to 107.0%. The method is simple, rapid, highly sensitive and with good repeatability. It could be used for the determination the BAs in the source water.

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