PMID: 11605763Oct 19, 2001Paper

Determination of flavonoids and stilbenes in red wine and related biological products by HPLC and HPLC-ESI-MS-MS

Fresenius' Journal of Analytical Chemistry
G StecherG K Bonn

Abstract

To investigate probable health benefits of flavonoids and stilbenes in red wine a new reversed-phase (RP) high-performance liquid-chromatographic (HPLC) method with enhanced separation efficiency and improved selectivity, sensitivity, and speed has been established for determination of the flavonoids quercetin, myricetin and kaempferol and the stilbenes cis- and trans-resveratrol, in a single run . UV-absorbance, fluorescence (FLD), and mass-spectrometric (MS) detection were also evaluated. UV-absorbance detection at 320 nm for stilbenes and 377 nm for flavonoids enables their determination up to the nanogram range with a linearity of R2>0.9999 (linear range 50 ng mL(-1)-50 microg mL(-1)). Calculated values of average recoveries were between 95 and 105% for all analytes. For resveratrol, fluorescence detection was highly selective and twice as sensitive as UV detection, and linearity was satisfactory (R2>0.9996; linear range see UV detection). For the detection of the hydrophilic glycosidic compounds piceid and rutin, which are coeluted with other hydrophilic ingredients, the validated RP HPLC system was coupled to a quadrupole ion-trap mass-spectrometer (MS) via an electrospray interface (ESI) with 25% ammonia solution as shea...Continue Reading

Citations

Dec 21, 2006·Analytical and Bioanalytical Chemistry·T Galeano DíazD Airado Rodríguez
Nov 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dawei WenHuwei Liu
Jul 29, 2008·Plant Physiology and Biochemistry : PPB·Daniel K OwensBrenda S J Winkel
Dec 2, 2005·Journal of Separation Science·Amparo ChaferAngel Berna
Aug 7, 2007·Rapid Communications in Mass Spectrometry : RCM·Francesca BuiarelliAlessandro Terracciano
Mar 14, 2008·Journal of Separation Science·Laura MercoliniMaria Augusta Raggi
May 23, 2013·Biomedical Chromatography : BMC·Haojing SongRuichen Guo
Sep 25, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Kelly Carolina Frauzino AraújoValéria de Oliveira
Feb 7, 2014·Critical Reviews in Food Science and Nutrition·Karl FraserSusanne Rasmussen
Oct 27, 2016·International Journal of Molecular Sciences·Julia López-Hernández, Ana Rodríguez-Bernaldo de Quirós
Mar 29, 2002·Journal of Mass Spectrometry : JMS
Nov 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jun WangGiulio Maria Pasinetti
Feb 1, 2007·Electrophoresis·Stefan BachmannGünther K Bonn
May 9, 2006·Acta Biochimica Et Biophysica Sinica·Lei FengWen-Yi Tao
Apr 30, 2008·Journal of Agricultural and Food Chemistry·Tahira SultanaGuenther Karl Bonn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.