Determination of florfenicol amine residues in animal edible tissues by an indirect competitive ELISA

Journal of Agricultural and Food Chemistry
Jin-e WuDong-Ping He

Abstract

Florfenicol (FF) is a broad-spectrum antibiotic used increasingly in aquaculture, livestock, and poultry to treat diseases. To avoid using labor-intensive instrumental methods to detect residues of FF in food and food products, a simple and convenient indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method for florfenicol's major metabolite, florfenicol amine (FFA), was developed using a polyclonal antibody prepared in this study. FFA was covalently attached to carrier protein as immunogen by using the glutaraldehyde method. The antibodies obtained were characterized by an ELISA method and showed excellent specificity and sensitivity with the 50% inhibition values (IC 50) of 3.34 microg/L for FFA in PBS buffer. In the ELISA, sample extractions were performed by ethyl acetate/ammonium hydroxide (90 + 10, v/v) following combined acid hydrolysis of FF and its known metabolites. The limits of detection (LOD) calculated from the analysis of 20 known negative swine muscle, chicken muscle, and fish samples were 3.08, 3.3, and 3.86 microg/kg (mean + 3 SD), respectively. Recoveries of FFA fortified at the levels of 5, 50, 100, and 300 microg/kg ranged from 64.6 to 124.7%, with coefficients of variation of 11.3-25.8% ove...Continue Reading

References

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Citations

Jan 14, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·D R RezendeG L Rocha
May 24, 2013·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Xiaoqi TaoJianzhong Shen
Aug 8, 2012·Drug Testing and Analysis·Rodrigo H M M GranjaAmarylis C B A Wanschel
Jan 24, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haiyan ChenLan Ding
Dec 18, 2013·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Xiao S LiuXiao Y Zhang
Aug 17, 2018·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Mahdieh Sadat MohsenzadehSeyed Ahmad Mohajeri
Jul 19, 2013·Journal of Immunoassay & Immunochemistry·Shi-Yuan SheuTzong-Fu Kuo
Oct 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pengjie LuoJianzhong Shen
Sep 29, 2009·Journal of Chromatography. a·Brian KinsellaMartin Danaher

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