Determination of Fluorescence Quantum Yields in Scattering Media

Methods and Applications in Fluorescence
M Gabriela Lagorio

Abstract

The fluorescence quantum yield is a measure of the efficiency of photon emission and quantifies the luminescent performance of a given sample. The determination of fluorescence quantum yields, particularly in scattering media, is relevant in the areas of materials science, technology and photonics. It is equally crucial when studying fluorescent bioanalytical probes and biological systems either for medical applications, physiological analyses or the interpretation of optical signals in nature. This type of determination represents a challenge since light scattering introduces an appreciable complexity in the measurements. Hence, the use of experimentally accurate methods and the understanding of their basis and principles is indispensable for obtaining reliable results. In addition, light re-absorption processes are usually very significant in these systems and the experimental quantum yields normally differ from the true quantum yields of the fluorophore. The first purpose of this work is to provide a clear and comprehensive compilation of the various optical methods that can be used for the determination of quantum yields in scattering media. A second purpose is to present the correction models to account for light re-absorp...Continue Reading

References

Jan 15, 2005·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Luis F Vieira FerreiraAna M Botelho do Rego
May 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Martin MirendaEnrique San Román
Feb 16, 2006·Journal of Fluorescence·Laurent PorrèsAndrew Beeby
Aug 4, 2006·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Gabriela B Cordón, María G Lagorio
Sep 4, 2007·The Review of Scientific Instruments·Tai-Sang AhnChristopher J Bardeen
Aug 12, 2008·Current Protein & Peptide Science·Olesya V StepanenkoK K Turoverov
Mar 12, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Analia Iriel, María Gabriela Lagorio
Jun 6, 2013·Photochemistry and Photobiology·Hernán B Rodríguez, Enrique San Román
Jan 3, 2015·The Review of Scientific Instruments·S LeyreP Hanselaer
Mar 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Carlos TaboadaJulián Faivovich
Nov 30, 2018·Accounts of Chemical Research·Hernán B RodríguezEnrique San Román
May 1, 1976·Journal of Research of the National Bureau of Standards. Section A, Physics and Chemistry·J B Birks

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Citations

Sep 11, 2021·ACS Nano·Bradley J RyanMatthew G Panthani

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