Determination of fluoxetine in plasma by gas chromatography-mass spectrometry using stir bar sorptive extraction

Analytica Chimica Acta
Christian FernandesF M Lanças

Abstract

This article presents a method employing stir bar sorptive extraction (SBSE) with in situ derivatization, in combination with either thermal or liquid desorption on-line coupled to gas chromatography-mass spectrometry for the analysis of fluoxetine in plasma samples. Ethyl chloroformate was employed as derivatizing agent producing symmetrical peaks. Parameters such as solvent polarity, time for analyte desorption, and extraction time, were evaluated. During the validation process, the developed method presented specificity, linearity (R(2)>0.99), precision (R.S.D.<15%), and limits of quantification (LOQ) of 30 and 1.37 pg mL(-1), when liquid and thermal desorption were employed, respectively. This simple and highly sensitive method showed to be adequate for the measurement of fluoxetine in typical and trace concentration levels.

References

Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Adrián LLerenaMacarena Cáceres
Jan 29, 2003·Progress in Neuro-psychopharmacology & Biological Psychiatry·Meera VaswaniSubramanyam Ramesh
Jan 30, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cara PolsonRussell Grant
Dec 31, 2005·Journal of Chromatography. a·Alvaro J Santos NetoFernando M Lanças

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Citations

Sep 4, 2009·Analytical and Bioanalytical Chemistry·Hiroyuki Kataoka
Oct 19, 2013·Analytical and Bioanalytical Chemistry·Man HeBin Hu
Oct 2, 2009·Drug and Chemical Toxicology·Juliana de Castro-PradoMarialba A A de Castro-Prado
Jan 14, 2016·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Pavel SistikPetr Bednar
Dec 17, 2009·Journal of Pharmaceutical and Biomedical Analysis·Marina Salviato BalbãoMaria Eugênia Costa Queiroz
Jul 7, 2012·Biomedical Chromatography : BMC·Khalil FarhadiAmir Abbas Matin
Dec 15, 2010·Biomedical Chromatography : BMC·Prashant Laxman KoleRavi Sheshala
Feb 16, 2011·Paediatric Anaesthesia·Jeff S Millership
Sep 12, 2015·Bioanalysis·Chrysoula Nazyropoulou, Victoria Samanidou
Nov 26, 2010·Journal of Pharmaceutical and Biomedical Analysis·Prashant Laxman KoleJames C McElnay
Aug 11, 2016·Journal of Chromatographic Science·Zahra EslamiHassan Y Aboul-Enein
Mar 12, 2009·Journal of Separation Science·Fernando M LancasIgor R B Olivares
Jun 10, 2010·Journal of Separation Science·Sigrid MennickentC Gloria Godoy
Feb 8, 2011·Journal of Separation Science·Paulo Clairmont Feitosa de Lima GomesFernando Mauro Lancas
May 5, 2012·Journal of Pharmaceutical and Biomedical Analysis·Antônio Felipe Felicioni OliveiraAlvaro José Dos Santos-Neto
Jul 2, 2021·Analytical and Bioanalytical Chemistry·Noemí Molina-FernándezJon Sanz-Landaluze
May 30, 2015·Chemical Reviews·Justyna M Płotka-WasylkaJacek Namieśnik

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