Determination of fluvastatin enantiomers and the racemate in human blood plasma by liquid chromatography and fluorometric detection

Journal of Chromatography. a
H Toreson, B M Eriksson

Abstract

Liquid chromatographic methods for the determination of fluvastatin, as racemate and as separated enantiomers, are described. Fluvastatin was extracted at pH 6.0 from blood plasma into methyl tert.-butyl ether. The organic phase was evaporated and the extract redissolved into either a phosphate buffer solution of pH 6.0 containing tetrabutylammonium fluoride and methanol for the racemate determination, or in a mixture of acetonitrile and water for assaying the enantiomers. The absolute recoveries were 95 and 86% for the racemate and the enantiomers, respectively, and the limit of quantitation 0.5 nmol/1 for the racemate, and 5 nmol/l for the enantiomers, when using half a millilitre of plasma sample. The samples were chromatographed on a C8 column (racemate) and on a Chiralcel OD-R column (enantiomers), and monitored using fluorescence detection. In the achiral system, post-column exposure of the eluate to UV light enhanced the sensitivity by 4 to 5 times when compared with analysis based on the native fluorescence.

Citations

Sep 13, 2008·Archives of Pharmacal Research·Tran Quoc TrungKyeong Ho Kim
Aug 9, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sidika ErtürkSevil Müge Cetin
Aug 28, 1998·The Journal of Pharmacy and Pharmacology·A LindahlH Lennernäs
Sep 18, 2001·Biomedical Chromatography : BMC·D Doğrukol-AkH Y Aboul-Enein
May 10, 2006·Journal of Pharmaceutical and Biomedical Analysis·So Young UmSun Ok Choi
Apr 13, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mariusz StolarczykJan Krzek
Aug 18, 2001·Journal of Cardiovascular Pharmacology and Therapeutics·R SiekmeierW Jaross
Nov 1, 1996·Clinical Pharmacology and Therapeutics·A LindahlH Lennernäs

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.