Abstract
An analytical method for gentamicin in animal tissues was developed and validated. An alkaline mobile phase with an HPH C8 column was selected so that all the four gentamicin components were retained and eluted without using fluorinated ion-pairing reagents. The method is sufficiently sensitive and highly selective, using a strong cation-exchange solid-phase extraction cartridge (PCX) to clean up the samples. Different types of solid-phase extraction columns and membranes were considered to obtain a high recovery. The method was validated on spiking samples, recovery, inter- and intra-assay variation, to ensure its accuracy and precision. The LOQ (S/N ≥ 10) for gentamicin in goat meat, liver, kidney and adipose tissue was 25, 50, 30 and 30 ng/g, respectively; the LOD (S/N ≥ 3) was 5, 10, 10 and 10 ng/g, respectively. The recoveries were between 88% and 106%. The method in all animal tissues was calibrated from 10 to 1000 μg/L in the matrix-assisted standard solution. The novelty of this method is that the commonly used fluorinated ion-pairing reagent was not used in the mobile phase in our analysis, greatly reducing the contamination of the ESI source in negative mode. Moreover, the four gentamicin components were clearly separ...Continue Reading
References
Dec 13, 2000·Journal of Mass Spectrometry : JMS·M CherletP D Backer
May 19, 2001·Journal of Chromatography. a·A PosyniakJ Niedzielska
Apr 9, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Abdulsalam I Al-AmoudHenry Chrystyn
Feb 6, 2003·Rapid Communications in Mass Spectrometry : RCM·D DebremaekerJ Hoogmartens
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Jul 25, 2003·Journal of Chromatography. a·Dirk Löffler, Thomas A Ternes
Feb 4, 2005·Electrophoresis·Eliangiringa KaaleJos Hoogmartens
May 21, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David N HellerKeesla Moulton
Nov 1, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David N HellerO Alberto Chiesa
Sep 2, 2008·Journal of Chromatography. a·Wei-xia ZhuShu-sheng Zhang
Mar 17, 2009·Analytica Chimica Acta·Frédérique L van HolthoonMatthew Sharman
Aug 4, 2009·Journal of Agricultural and Food Chemistry·Xiangmei LiXilong Xiao
Feb 5, 2010·Journal of Agricultural and Food Chemistry·Perry A MartosBryn Shurmer
Dec 14, 2011·Analytica Chimica Acta·A KaufmannK Maden
Aug 8, 2012·Food Chemistry·Yanfei TaoZonghui Yuan
Oct 1, 2013·Journal of Chromatography. a·Steven J LehotayLouis Bluhm
May 25, 2016·Journal of Chromatography. a·Marilena E DasenakiNikolaos S Thomaidis
Aug 20, 2016·Rapid Communications in Mass Spectrometry : RCM·Liusheng FanWei Xu
Aug 9, 2017·Journal of AOAC International·Bixia YangChengjun Sun
Mar 3, 2018·Journal of Chromatography. a·Xinran WangJinhui Zhou
Citations
Jun 24, 2020·Phytochemical Analysis : PCA·Qiulin WangYan Qiu
Feb 2, 2021·Comprehensive Reviews in Food Science and Food Safety·Ancuta MogaErnesto Francisco Simó-Alfonso
Sep 3, 2021·Food Chemistry·Wei JiaLin Shi
Sep 3, 2021·Rapid Communications in Mass Spectrometry : RCM·Zhengsheng MaoFeng Chen