Determination of free fatty acids in plasma by gas chromatography.

Analytical Biochemistry
Ken'ichi IchiharaTakehiro Masumura

Abstract

A method was developed for determination of free fatty acids (FFAs) in plasma by gas chromatography. Plasma was extracted with 3 vol of methanol. Most cholesterol esters and triacylglycerols did not dissolve in the aqueous methanol. FFAs in the crude lipid solution were directly and selectively methylated with (trimethylsilyl)diazomethane at room temperature. Fatty acid methyl esters (FAMEs) formed were extracted with hexane, and nonreactive phospholipids were washed out with 95% methanol. The partially purified FAME preparation was analyzed by gas chromatography. The composition and amount of plasma FFAs closely approximated those obtained using two different methods.

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Citations

Nov 25, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hector Gallart-AyalaJulijana Ivanisevic
Jun 25, 2021·Advanced Science·Joanne X ShenVolker M Lauschke

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