Determination of fungicide residues in fruits by coupled-column liquid chromatography

Journal of Separation Science
T ZamoraF Hernández

Abstract

Coupled-column liquid chromatography with fluorescence detection was applied to the determination of o-phenylphenol and bitertanol residues in orange and banana fruits. After extraction with a mixture of acetone, dichloromethane-petroleum ether, and ethyl acetate, an extract aliquot of 100 microL was injected directly without any additional clean-up into the chromatographic system using two reversed phase C18 coupled columns. The LC-LC approach allowed automated sample clean up of the vegetal extracts, leading to a simple and rapid analytical procedure, with limits of quantification between 0.01 and 0.05 mg kg(-1). Recovery experiments performed on orange and banana samples fortified at different concentrations (0.01 - 4 mg kg(-1)) gave average recoveries between 70 and 113% with relative standard deviations lower than 15%. The procedure developed was finally applied to orange and banana samples from different geographical locations and the results were confirmed by GC-MS.

References

Aug 28, 2001·Journal of Chromatography. a·R RodríguezJ Mañes

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Citations

May 9, 2012·Environmental Science and Pollution Research International·Virgínia C FernandesCristina Delerue-Matos
Jan 22, 2009·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kunihiko TakahashiYoshihumi Hirose

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