Determination of gamma/delta and other T-lymphocyte subsets in bronchoalveolar lavage fluid and peripheral blood from patients with sarcoidosis and idiopathic fibrosis of the lung

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
R GruberG Riethmüller

Abstract

We measured five different lymphocyte subpopulations, including gamma/delta-T cells, in peripheral blood and bronchoalveolar lavage (BAL) fluid from 48 consecutive patients undergoing diagnostic BAL (patients with sarcoidosis (n = 19), patients with idiopathic pulmonary fibrosis (IPF; n = 11) and patients with other diseases of the lung). Quantitative analysis of CD3+, CD4+, CD8+, CD16/56+ cells and gamma/delta-T cells was done by flow cytometry. The proportion of CD3+ and CD4+ cells in the peripheral blood of patients with sarcoidosis and IPF was significantly diminished, while CD8+ lymphocytes and NK cells were significantly elevated compared to healthy controls. There was no significant difference for the gamma/delta-T cell subpopulation between patients with sarcoidosis, IPF and control group. The proportion of CD3+ and CD4+ cells in BAL fluid was significantly elevated in sarcoidosis compared to IPF, while CD8+ cells were significantly diminished. Natural killer cells, defined as CD16/56+ CD3- cells, showed comparable low numbers in sarcoidosis and IPF. For gamma/delta-T cells no significant difference was found between patients with sarcoidosis and IPF.

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Citations

Aug 17, 2000·Springer Seminars in Immunopathology·C FonsecaC M Black
Dec 12, 2001·Survey of Ophthalmology·S R BoydS Lightman
Mar 1, 2003·Pathology International·Mohammed S Razzaque, Takashi Taguchi
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Jan 29, 2010·Trends in Immunology·Georg WickRoswitha Sgonc
Apr 17, 2014·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Siri T LehtonenRiitta Kaarteenaho

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