PMID: 8596699Feb 1, 1996Paper

Determination of gap junctional intercellular communication by capacitance measurements

Pflügers Archiv : European journal of physiology
A D de RoosA P Theuvenet

Abstract

Electrical coupling between cells is usually measured using the double-patch-clamp technique with cell pairs. Here, a single patch-clamp technique that is not limited to cell pairs is described to determine electrical coupling between cells. Capacitance measurements in clusters of normal rat kidney (NRK) fibroblasts were used to study intercellular communication. In the whole-cell patch-clamp configuration capacitive transients were evoked by applying small voltage pulses. Total membrane capacitance was calculated from these capacitive transients after determination of access resistance, membrane conductance, and the decay constant of the transients, or alternatively by integrating the current transient. We found that in clusters of one to ten cells, membrane capacitance increased linearly with cell number, showing that the cells are electrically coupled. Membrane conductance of the cluster of cells also increased, as expected for cells that are well coupled. In subconfluent and confluent cultures, high membrane conductances together with large capacitive transients were observed, indicative of electrical coupling. Capacitance could only be determined qualitatively under these conditions, due to space clamp problems. In the pre...Continue Reading

References

Nov 28, 1979·Biochemical and Biophysical Research Communications·A W Murray, D J Fitzgerald
Jan 21, 1977·Science·I SimpsonW R Loewenstein
Jan 1, 1992·Methods in Enzymology·C M Armstrong, W F Gilly
Jun 1, 1992·The American Journal of Physiology·B R Takens-KwakA C Van Ginneken
May 1, 1991·Pflügers Archiv : European journal of physiology·R M SantosM Valdeolmillos
Jan 1, 1990·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·J E TroskoJ E Klaunig
Sep 2, 1985·Nature·J Neyton, A Trautmann
Dec 1, 1988·The Journal of Membrane Biology·P E MaldonadoW R Loewenstein
Jan 1, 1985·Annual Review of Physiology·D C Spray, M V Bennett
Feb 1, 1988·Pflügers Archiv : European journal of physiology·M Lindau, E Neher
Jul 1, 1988·The Journal of Cell Biology·G H Kalimi, C W Lo
Jan 1, 1981·The Journal of General Physiology·D C SprayM V Bennett
Oct 1, 1981·Physiological Reviews·W R Loewenstein
Apr 29, 1982·Biochimica Et Biophysica Acta·M L Hooper
Nov 1, 1994·In Vitro Cellular & Developmental Biology. Animal·D T KiangM M Atkinson
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L BrissetteG P Dotto
Jan 1, 1993·Methods in Enzymology·R T Kado

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Citations

Feb 20, 2013·European Journal of Pharmacology·Xin-Zhi LiZhi-Gen Jiang
Feb 24, 2009·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Yoshimichi Yamamoto, Hikaru Suzuki
Jan 1, 2010·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Yoshimichi Yamamoto, Hikaru Suzuki
Nov 3, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Ke-Tao MaZhi-Gen Jiang
Feb 22, 2012·Biochimica Et Biophysica Acta·Jose F Ek-Vitorin, Janis M Burt
May 14, 2004·American Journal of Physiology. Cell Physiology·J J TorresD L Ypey
Jan 27, 2017·American Journal of Physiology. Cell Physiology·Lise HangaardVladimir V Matchkov
Aug 2, 2003·Journal of Cellular Physiology·E G A HarksA P R Theuvenet
Jan 24, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Vladimir V MatchkovChristian Aalkjaer
Jun 2, 2006·American Journal of Respiratory and Critical Care Medicine·David J Erle, Guohua Zhen
Sep 13, 2002·American Journal of Physiology. Cell Physiology·N Yvonne Tallini, Larry C Stoner
Jan 29, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Alessia OrlandiMaurizio C Capogrossi
Mar 11, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Erik G A HarksAlexander P R Theuvenet
Jan 22, 1998·The American Journal of Physiology·J W Stelling, T J Jacob
May 9, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Vladimir V MatchkovChristian Aalkjaer
May 1, 1997·Current Eye Research·K K Williams, M A Watsky
Jan 22, 1998·The American Journal of Physiology·A De RoosA P Theuvenet
Jun 16, 1999·Journal of Neurophysiology·P V NguyenP Drapeau
Apr 26, 2018·PloS One·Alexandra V Ulyanova, Roman E Shirokov
Sep 13, 2005·Development·Ann M FlennikenJanet Rossant

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