Determination of gene expression patterns by in situ hybridization in sections

Methods in Molecular Biology
Kateryna KyrylkovaMark Leid

Abstract

In recent years, in situ RNA hybridization technique has found a widespread application in developmental biology. This method has frequently been used to determine gene expression patterns, which is a first step toward understanding of a gene function. Here, we provide a reliable and sensitive method for in situ RNA hybridization on frozen sections of mouse embryo using digoxigenin-labeled RNA probes. This technique can be used to study gene expression patterns at all stages of odontogenesis.

Citations

Oct 11, 2015·Developmental Biology·Kateryna KyrylkovaMark Leid
Jan 31, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hsiao-Yen MaChrissa Kioussi

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