Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay

Pesticide Biochemistry and Physiology
Recep LimanNur Serap Öztürk

Abstract

Imazethapyr (IM) is an imidazolinone herbicide that is currently used for broad-spectrum weed control in soybean and other legume crops. In this study, cytotoxic and genotoxic effects of IM were investigated by using mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs) and DNA damage on the root meristem cells of Allium cepa. In Allium root growth inhibition test, EC50 value was determined as 20 ppm, and 0.5xEC50, EC50 and 2xEC50 concentrations of IM herbicide were introduced to onion tuber roots. Distilled water and methyl methane sulfonate (MMS, 10 mg/L) were used as a negative and positive control, respectively. As A. cepa cell cycle is 24 hours, so, application process was carried out for 24, 48, 72 and 96 hours. All the applied doses decreased MIs compared to control group and these declines were found to be statistically meaningful. Analysis of the chromosomes showed that 10 ppm IM except for 48 h induced CAs but 40 ppm IM except for 72 h decreased CAs. DNA damage was found significantly higher in 20 and 40 ppm of IM compared to the control in comet assay. These results indicated that IM herbicide exhibits cytotoxic activity but not genotoxic activity (except 10 ppm) and induced DNA damage in a dose depende...Continue Reading

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Citations

Aug 8, 2015·Environmental Science and Pollution Research International·Anahí MagdalenoÁngela B Juárez
May 8, 2015·Cytotechnology·Maruhen Amir Datsch SilveiraLuciana Paula Grégio d'Arce
Oct 26, 2018·Environmental Science and Pollution Research International·Derya Küçük, Recep Liman
Jan 15, 2020·Bulletin of Environmental Contamination and Toxicology·Recep LimanMeltem Demirel Kars
Dec 22, 2019·Foods·Jesús Enrique Chan-HigueraAngel Antonio Carbonell-Barrachina
Mar 9, 2017·Environmental Science and Pollution Research International·Sonia SoloneskiMarcelo L Larramendy
Jan 2, 2019·International Journal of Environmental Research and Public Health·Catalin Aurelian RosculeteLiviu Aurel Olaru
May 14, 2020·Journal of Applied Genetics·Gamze Aydın, Recep Liman
Mar 23, 2018·Environmental Monitoring and Assessment·Sonam Verma, Alka Srivastava
Apr 15, 2019·Environmental Geochemistry and Health·Raylane Pereira GomesLilian Carla Carneiro
Apr 4, 2020·Environmental Science and Pollution Research International·Damla Selin Çıldır, Recep Liman
Jan 21, 2021·Environmental Monitoring and Assessment·Adiba KhanPraveen C Verma
Feb 3, 2021·Environmental Science and Pollution Research International·Jyoti RanjanDalia Dasgupta Mandal
Apr 17, 2021·Environmental Science and Pollution Research International·Recep LimanCengiz Sarıkurkcu
Apr 30, 2021·Environmental Science and Pollution Research International·Eslem Amaç, Recep Liman
May 24, 2021·Environmental Science and Pollution Research International·Mariyana GeorgievaValya Vassileva
Jun 17, 2021·Brazilian Journal of Biology = Revista Brasleira De Biologia·N C GhisiE C Oliveira

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