PMID: 9174274Apr 11, 1997Paper

Determination of gentamicin in bovine milk using liquid chromatography with post-column derivatization and fluorescence detection

Journal of Chromatography. B, Biomedical Sciences and Applications
P J KijakB Shaikh

Abstract

A method capable of separating and quantifying the three major and one minor components of gentamicin in milk has been developed. The method is capable of detecting 15 ng/ml gentamicin, based on a total of the four components. Milk samples are centrifuged at 4 degrees C, the fat layer removed, and the samples deproteinated with 30% trichloracetic acid. After a second centrifugation, the supernatant is passed through a C18 solid-phase extraction column. The column is washed with water, water-methanol (50:50) and methanol. Ammonium hydroxide (16%) in methanol is used to elute the gentamicin. The eluent is evaporated to near dryness and taken up with water. An aliquot of the sample is then mixed with an ion-pairing reagent for chromatography. Separation is achieved using pentanesulfonic acid in a water-methanol mobile phase on a C18 reversed-phase column. The o-phthalaldehyde fluorescence derivatives of gentamicin are formed post-column and are detected with excitation at 340 nm and emission at 430 nm. The percent recovery of gentamicin averaged 72, 78 and 88% from milk samples fortified at 15, 30 and 60 ng/ml, respectively.

Citations

Apr 9, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Abdulsalam I Al-AmoudHenry Chrystyn
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·R IshiiJ MacNeil
Dec 1, 1995·Journal of Veterinary Pharmacology and Therapeutics·W M PedersoliR A Frobish
Jul 18, 2008·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Hiroko KajitaTakashi Komukai
Apr 7, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sherri B TurnipseedMark R Madson
Sep 8, 2004·Journal of Pharmaceutical and Biomedical Analysis·Nikolaos C Megoulas, Michael A Koupparis
Oct 22, 2008·Critical Reviews in Biotechnology·C Ganesh KumarAnnapurna Jetty
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·D A Stead
Aug 6, 1998·Journal of Chromatography. a·F J Schenck, P S Callery

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