Determination of Giardia duodenalis assemblages and multi-locus genotypes in patients with sporadic giardiasis from England

Parasites & Vectors
Corrado MinettiJonathan M Wastling

Abstract

The protozoan Giardia duodenalis is a common but highly diverse human parasite that comprises a complex of seven morphologically identical genetic assemblages, further divided into sub-assemblages. There is very little information available on the diversity of Giardia sub-assemblages and multi-locus genotypes infecting people in the United Kingdom. In this study we studied the molecular epidemiology of Giardia in symptomatic patients from North West England. Whole faecal DNA was extracted from the faecal samples of 406 Giardia cases and the parasites assemblage, sub-assemblage and multi-locus genotype were determined using PCR amplification, DNA sequencing and phylogenetic analysis of the beta-giardin, glutamate dehydrogenase, triose-phosphate isomerase and small-subunit ribosomal RNA genes. Information about age, gender and self-reported clinical outcomes was also collected from the patients to check for differences associated with the infecting Giardia assemblage. Our results showed a difference in the age prevalence of the two assemblages, with assemblage A being more common in older cases. Cases infected with assemblage B more often reported vomiting and a longer illness than cases infected with assemblage A. The majority o...Continue Reading

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Citations

Jul 3, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·A J AsherM L Power
Feb 10, 2018·Environmental Science and Pollution Research International·Meylin BautistaRegina Maura Bueno Franco
Aug 23, 2018·Paediatrics and International Child Health·Kapula Chifunda, Paul Kelly
Sep 8, 2019·Parasites & Vectors·Yuanfei WangLihua Xiao
Apr 11, 2019·Acta Parasitologica·Ehsanallah MirrezaieSomayeh Mirzavand
Jul 12, 2020·Genes·Adriana HigueraJuan David Ramírez
Aug 2, 2020·Parasites & Vectors·Carmen CostacheIoana Alina Colosi
Jan 5, 2017·Parasites & Vectors·Xiao-Xuan ZhangXing-Quan Zhu
May 22, 2021·Frontiers in Cellular and Infection Microbiology·Marwa ChourabiMarawan Abu-Madi
Oct 9, 2021·Parasitology Research·Weilong CaiYaoyu Feng

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Methods Mentioned

BETA
genotyping
PCR
enzyme-linked immunoassay
electrophoresis

Software Mentioned

Molecular Evolutionary Genetics Analysis ( MEGA )
MEGA
SPSS Statistics
BioEdit
Basic Local Alignment Search Tool ( BLAST )

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