Determination of glucosamine and N-acetyl glucosamine in fungal cell walls

Journal of Agricultural and Food Chemistry
Akram ZamaniMohammad J Taherzadeh

Abstract

A new method was developed to determine glucosamine (GlcN) and N-acetyl glucosamine (GlcNAc) in materials containing chitin and chitosan, such as fungal cell walls. It is based on two steps of hydrolysis with (i) concentrated sulfuric acid at low temperature and (ii) dilute sulfuric acid at high temperature, followed by one-step degradation with nitrous acid. In this process, chitin and chitosan are converted into anhydromannose and acetic acid. Anhydromannose represents the sum of GlcN and GlcNAc, whereas acetic acid is a marker for GlcNAc only. The method showed recovery of 90.1% of chitin and 85.7-92.4% of chitosan from commercial preparations. Furthermore, alkali insoluble material (AIM) from biomass of three strains of zygomycetes, Rhizopus oryzae, Mucor indicus, and Rhizomucor pusillus, was analyzed by this method. The glucosamine contents of AIM from R. oryzae and M. indicus were almost constant (41.7 +/- 2.2% and 42.0 +/- 1.7%, respectively), while in R. pusillus, it decreased from 40.0 to 30.0% during cultivation from 1 to 6 days. The GlcNAc content of AIM from R. oryzae and R. pusillus increased from 24.9 to 31.0% and from 36.3 to 50.8%, respectively, in 6 days, while it remained almost constant during the cultivation...Continue Reading

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Citations

Feb 14, 2012·Applied Biochemistry and Biotechnology·Rachma WikandariMohammad J Taherzadeh
Aug 22, 2013·Applied Biochemistry and Biotechnology·Marzieh MohammadiKeikhosro Karimi
Oct 9, 2012·Journal of Agricultural and Food Chemistry·Marzieh MohammadiKeikhosro Karimi
Dec 15, 2010·International Journal of Molecular Sciences·Akram ZamaniMohammad J Taherzadeh
Jun 21, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rani AbroTorbjörn Lundh
Jul 25, 2015·International Journal of Molecular Sciences·Zahra SafaeiAkram Zamani
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Aug 25, 2010·Analytical Biochemistry·Yu-Shen ChengJean S VanderGheynst
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