Determination of glutathione in apoptotic SMMC-7221 cells induced by xylitol selenite using capillary electrophoresis

Biotechnology Letters
Xue WuChao Qi

Abstract

To determine the glutathione (GSH) content in a human hepatoma cell line (SMMC-7221) treated with xylitol/selenite, providing a part of an investigation of its anti-cancer mechanisms. The nuclei of SMMC-7221 cells were stained with Hoechst 33258 in an apoptosis assay, and their morphology subsequently changed from circular to crescent shape. The calibration curve (r(2) = 0.992) was established, and GSH content markedly decreased after treated with 0.5 and 1 mg xylitol/selenite l(-1) for 12, 36 and 60 h (12 h: from 95.57 ± 19.57 to 29.09 ± 7.74 and 24.27 ± 11.15; 36 h: from 70.73 ± 11.35 to 19.54 ± 6.39 and 9.35 ± 6.69; 60 h: from 72.63 ± 16.94 to 7.432 ± 3.84 and 0). The depletion rate of GSH was more related to the concentration of xylitol/selenite than the treatment time (from 69.95 ± 1.87 to 100 % vs. 0.22 ± 0.2 to 100 %). Xylitol/selenite is a promising anti-cancer drug to induce apoptosis in SMMC-7221 cells. It may regulate the apoptosis through the co-action of multiple mechanisms related to GSH depletion.

References

Jun 13, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jeffrey S Armstrong, Dean P Jones
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Emanuela Camera, Mauro Picardo
Mar 9, 2004·Electrophoresis·Ciriaco CarruAngelo Zinellu
Jun 10, 2004·Electrophoresis·Christophe BayleFrançois Couderc
Jun 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tsutomu SatoYoshiro Niitsu
Jan 5, 2007·Analytical and Bioanalytical Chemistry·Alessandro MusengaMaria Augusta Raggi
Jun 2, 2007·The Journal of Biological Chemistry·Miguel A AonBrian O'Rourke
Jun 5, 2012·Antioxidants & Redox Signaling·Rodrigo Franco, John A Cidlowski

❮ Previous
Next ❯

Citations

Nov 13, 2018·Natural Product Research·Samit BeraAmalesh Samanta
Sep 10, 2016·Electrophoresis·Júlia LačnáPetr Kubáň
Jul 17, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Xueling ShanZhidong Chen
Mar 8, 2020·Inflammopharmacology·Sneha Hariharan, Selvakumar Dharmaraj
Sep 21, 2017·Oncology Letters·Qiang YuShichun Lu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Related Papers

Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban
Zhongzhi TangLibo Fu
International Journal of Clinical and Experimental Medicine
Yuxiang FanHongliang Zhang
© 2022 Meta ULC. All rights reserved